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PMID: 12703773 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

First in vitro directed biosynthesis of new compounds by a minimal type II polyketide synthase: evidence for the mechanism of chain length determination.

Chemical communications (Cambridge, England) ·No. 6 ·2003-03-21 ·Pages 686-7

Nicholson TP, Winfield C, Westcott J, Crosby J, Simpson TJ, Cox RJ

Abstract

The minimal actinorhodin polyketide synthase bearing two point mutations (KSbeta Q161A, ACP C17S) was chemically modified to carry novel C4 to C8 starter units on the ACP: on incubation with an excess of malonyl CoA new 16-carbon polyketides are made, supporting a measuring mechanism.

MeSH Terms

Acyl Carrier Protein/metabolism Acyltransferases/chemistry,genetics,metabolism Anthraquinones/chemistry,metabolism Bacterial Proteins Malonyl Coenzyme A/metabolism Polyketide Synthases Spectrometry, Mass, Electrospray Ionization Streptomyces/enzymology

Chemicals

Acyl Carrier Protein Anthraquinones Bacterial Proteins Malonyl Coenzyme A Polyketide Synthases Acyltransferases polyketide beta-ketoacylsynthase actinorhodin

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Nicholson T P

School of Chemistry, University of Bristol, Cantock's Close, Bristol, UK BS8 1TS.

Winfield C

Westcott J

Crosby J

Simpson T J

Cox R J

Article Info

Journal

Chemical communications (Cambridge, England)

Abbr.

Chem Commun (Camb)

ISSN

1359-7345

DOI

10.1039/b300847a

Published

2003-03-21

Pages

686-7

Language

English

Region

England

NLM ID

9610838

Subset

External Links

PubMed source DOI: 10.1039/b300847a View journal details

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First in vitro directed biosynthesis of new compounds by a minimal type II polyketide synthase: evidence for the mechanism of chain length determination.

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