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PMID: 12702673 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Chromosomal lesion suppression and removal in Escherichia coli via linear DNA degradation.

Genetics ·Vol. 163 ·No. 4 ·2003-04-00 ·Pages 1255-71

Miranda A, Kuzminov A

Abstract

RecBCD is a DNA helicase/exonuclease implicated in degradation of foreign linear DNA and in RecA-dependent recombinational repair of chromosomal lesions in E. coli. The low viability of recA recBC mutants vs. recA mutants indicates the existence of RecA-independent roles for RecBCD. To distinguish among possible RecA-independent roles of the RecBCD enzyme in replication, repair, and DNA degradation, we introduced wild-type and mutant combinations of the recBCD chromosomal region on a low-copy-number plasmid into a DeltarecA DeltarecBCD mutant and determined the viability of resulting strains. Our results argue against ideas that RecBCD is a structural element in the replication factory or is involved in RecA-independent repair of chromosomal lesions. We found that RecBCD-catalyzed DNA degradation is the only activity important for the recA-independent viability, suggesting that degradation of linear tails of sigma-replicating chromosomes could be one of the RecBCD's roles. However, since the weaker DNA degradation capacity due a combination of the RecBC helicase and ssDNA-specific exonucleases restores viability of the DeltarecA DeltarecBCD mutant to a significant extent, we favor suppression of chromosomal lesions via linear DNA degradation at reversed replication forks as the major RecA-independent role of the RecBCD enzyme.

MeSH Terms
DNA/metabolism DNA Repair Escherichia coli/genetics Exodeoxyribonuclease V/genetics,metabolism Rec A Recombinases/genetics,metabolism
Chemicals
DNA Rec A Recombinases Exodeoxyribonuclease V
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Miranda Anabel
Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA.
Kuzminov Andrei
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/163.4.1255
Published
2003-04-00
Pages
1255-71
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1462524
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/163.4.1255 PMC Full Text View journal details
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