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PMID: 12697813 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The Tap42-protein phosphatase type 2A catalytic subunit complex is required for cell cycle-dependent distribution of actin in yeast.

Molecular and cellular biology ·Vol. 23 ·No. 9 ·2003-05-00 ·Pages 3116-25

Wang H, Jiang Y

Abstract

In Saccharomyces cerevisiae, the Tor proteins mediate a wide spectrum of growth-related cellular processes in response to nutrients. The pleiotropic role of the Tor proteins is mediated, at least in part, by type 2A protein phosphatases (PP2A) and 2A-like protein phosphatases. Tor-mediated signaling activity promotes the interaction of phosphatase-interacting protein Tap42 with PP2A and 2A-like protein phosphatases. The distinct complexes formed between Tap42 and different phosphatases mediate various cellular events and modulate phosphorylation levels of many downstream factors in the Tor pathway in a Tor-dependent and rapamycin-sensitive manner. In this study, we demonstrate that the interaction between Tap42 and the catalytic subunits of PP2A (PP2Ac) is required for cell cycle-dependent distribution of actin. We show that mutations in PP2Ac and Tap42 that perturb the interaction cause random distribution of actin during the cell cycle and that overexpression of the Rho2 GTPase suppresses the actin defects associated with the mutants. Our findings suggest that the Tap42-PP2Ac complex regulates the actin cytoskeleton via a Rho GTPase-dependent mechanism. In addition, we provide evidence that PP2A activity plays a negative role in controlling the actin cytoskeleton and, possibly, in regulation of the G(2)/M transition of the cell cycle.

MeSH Terms
Actins/metabolism Adaptor Proteins, Signal Transducing Catalytic Domain Cell Cycle/physiology Cell Polarity Cytoskeleton/drug effects,genetics,metabolism Gene Expression Regulation, Fungal Macromolecular Substances Monomeric GTP-Binding Proteins Mutation Phosphoprotein Phosphatases/genetics,metabolism Protein Subunits Saccharomyces cerevisiae/cytology,drug effects,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Sirolimus/pharmacology rho GTP-Binding Proteins
Chemicals
Actins Adaptor Proteins, Signal Transducing Macromolecular Substances Protein Subunits Saccharomyces cerevisiae Proteins TAP42 protein, S cerevisiae Phosphoprotein Phosphatases Monomeric GTP-Binding Proteins RHO2 protein, S cerevisiae rho GTP-Binding Proteins Sirolimus
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wang Huamin
Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA.
Jiang Yu
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.23.9.3116-3125.2003
Published
2003-05-00
Pages
3116-25
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC153200
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.23.9.3116-3125.2003 PMC Full Text View journal details
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