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PMID: 12682026 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The yeast Sgs1 helicase is differentially required for genomic and ribosomal DNA replication.

The EMBO journal ·Vol. 22 ·No. 8 ·2003-04-15 ·Pages 1939-49

Versini G, Comet I, Wu M, Hoopes L, Schwob E, Pasero P

Abstract

The members of the RecQ family of DNA helicases play conserved roles in the preservation of genome integrity. RecQ helicases are implicated in Bloom and Werner syndromes, which are associated with genomic instability and predisposition to cancers. The human BLM and WRN helicases are required for normal S phase progression. In contrast, Saccharomyces cerevisiae cells deleted for SGS1 grow with wild-type kinetics. To investigate the role of Sgs1p in DNA replication, we have monitored S phase progression in sgs1Delta cells. Unexpectedly, we find that these cells progress faster through S phase than their wild-type counterparts. Using bromodeoxyuridine incorporation and DNA combing, we show that replication forks are moving more rapidly in the absence of the Sgs1 helicase. However, completion of DNA replication is strongly retarded at the rDNA array of sgs1Delta cells, presumably because of their inability to prevent recombination at stalled forks, which are very abundant at this locus. These data suggest that Sgs1p is not required for processive DNA synthesis but prevents genomic instability by coordinating replication and recombination events during S phase.

MeSH Terms
Bromodeoxyuridine/metabolism Cell Cycle/physiology Cell Cycle Proteins Checkpoint Kinase 2 DNA Helicases/genetics,metabolism DNA Replication DNA, Ribosomal/genetics,metabolism DNA-Binding Proteins/metabolism Humans Protein Serine-Threonine Kinases/metabolism Rad52 DNA Repair and Recombination Protein RecQ Helicases Saccharomyces cerevisiae/physiology Saccharomyces cerevisiae Proteins/genetics,metabolism
Chemicals
Cell Cycle Proteins DNA, Ribosomal DNA-Binding Proteins FOB1 protein, S cerevisiae RAD52 protein, S cerevisiae Rad52 DNA Repair and Recombination Protein Saccharomyces cerevisiae Proteins Checkpoint Kinase 2 Protein Serine-Threonine Kinases RAD53 protein, S cerevisiae SGS1 protein, S cerevisiae DNA Helicases RecQ Helicases Bromodeoxyuridine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Versini Gwennaelle
Institute of Molecular Genetics, CNRS and Université Montpellier II, 1919 route de Mende, 34293 Montpellier, France.
Comet Itys
Wu Michelle
Hoopes Laura
Schwob Etienne
Pasero Philippe
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdg180
Published
2003-04-15
Pages
1939-49
Language
English
Region
England
NLM ID
8208664
PMCID
PMC154472
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/cdg180 PMC Full Text View journal details
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