Home LiteratureArticle Details
PMID: 12676708 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Bacterioplankton community shifts in an arctic lake correlate with seasonal changes in organic matter source.

Applied and environmental microbiology ·Vol. 69 ·No. 4 ·2003-04-00 ·Pages 2253-68

Crump BC, Kling GW, Bahr M, Hobbie JE

Abstract

Seasonal shifts in bacterioplankton community composition in Toolik Lake, a tundra lake on the North Slope of Alaska, were related to shifts in the source (terrestrial versus phytoplankton) and lability of dissolved organic matter (DOM). A shift in community composition, measured by denaturing gradient gel electrophoresis (DGGE) of 16S rRNA genes, occurred at 4 degrees C in near-surface waters beneath seasonal ice and snow cover in spring. This shift was associated with an annual peak in bacterial productivity ([(14)C]leucine incorporation) driven by the large influx of labile terrestrial DOM associated with snow meltwater. A second shift occurred after the flux of terrestrial DOM had ended in early summer as ice left the lake and as the phytoplankton community developed. Bacterioplankton communities were composed of persistent populations present throughout the year and transient populations that appeared and disappeared. Most of the transient populations could be divided into those that were advected into the lake with terrestrial DOM in spring and those that grew up from low concentrations during the development of the phytoplankton community in early summer. Sequencing of DNA in DGGE bands demonstrated that most bands represented single ribotypes and that matching bands from different samples represented identical ribotypes. Bacteria were identified as members of globally distributed freshwater phylogenetic clusters within the alpha- and beta-Proteobacteria, the Cytophaga-Flavobacteria-Bacteroides group, and the ACTINOBACTERIA:

MeSH Terms
Animals Arctic Regions Bacteria/classification,genetics,isolation & purification DNA, Ribosomal/analysis Ecosystem Electrophoresis Fresh Water/microbiology Molecular Sequence Data Organic Chemicals/metabolism Plankton RNA, Ribosomal, 16S/genetics Ribotyping Seasons Sequence Analysis, DNA
Chemicals
DNA, Ribosomal Organic Chemicals RNA, Ribosomal, 16S
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Crump Byron C
The Ecosystems Center, Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA. bcrump@mbl.edu
Kling George W
Bahr Michele
Hobbie John E
References (20)
20 references, click to expand
  1. Dynamics of bacterial community composition and activity during a mesocosm diatom bloom.
    Appl Environ Microbiol. 2000 Feb;66(2):578-87 PMID: 10653721
  2. Comparative 16S rRNA analysis of lake bacterioplankton reveals globally distributed phylogenetic clusters including an abundant group of actinobacteria.
    Appl Environ Microbiol. 2000 Nov;66(11):5053-65 PMID: 11055963
  3. Genetic diversity in Sargasso Sea bacterioplankton.
    Nature. 1990 May 3;345(6270):60-3 PMID: 2330053
  4. Spatial differences in bacterioplankton composition along the Catalan coast (NW Mediterranean) assessed by molecular fingerprinting.
    FEMS Microbiol Ecol. 2000 Jul 1;33(1):51-59 PMID: 10922503
  5. Phylogenetic diversity of subsurface marine microbial communities from the Atlantic and Pacific Oceans.
    Appl Environ Microbiol. 1993 May;59(5):1294-302 PMID: 7685997
  6. Detritus-dependent development of the microbial community in an experimental system: qualitative analysis by denaturing gradient gel electrophoresis.
    Appl Environ Microbiol. 1999 Jun;65(6):2478-84 PMID: 10347030
  7. Seasonal dynamics of bacterioplankton community structure in a eutrophic lake as determined by 5S rRNA analysis.
    Appl Environ Microbiol. 1999 Jul;65(7):3164-74 PMID: 10388718
  8. Bacterioplankton Production in Humic Lake Örträsket in Relation to Input of Bacterial Cells and Input of Allochthonous Organic Carbon.
    Microb Ecol. 2000 Feb;39(2):101-115 PMID: 10833223
  9. Use of nuclepore filters for counting bacteria by fluorescence microscopy.
    Appl Environ Microbiol. 1977 May;33(5):1225-8 PMID: 327932
  10. Changes in bacterial and eukaryotic community structure after mass lysis of filamentous cyanobacteria associated with viruses.
    Appl Environ Microbiol. 1999 Feb;65(2):795-801 PMID: 9925618
  11. Phylogenetic analysis of particle-attached and free-living bacterial communities in the Columbia river, its estuary, and the adjacent coastal ocean.
    Appl Environ Microbiol. 1999 Jul;65(7):3192-204 PMID: 10388721
  12. Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA.
    Appl Environ Microbiol. 1997 Nov;63(11):4516-22 PMID: 9361437
  13. Nearly identical 16S rRNA sequences recovered from lakes in North America and Europe indicate the existence of clades of globally distributed freshwater bacteria.
    Syst Appl Microbiol. 1998 Dec;21(4):546-56 PMID: 9924823
  14. Effect of PCR conditions on the formation of heteroduplex and single-stranded DNA products in the amplification of bacterial ribosomal DNA spacer regions.
    PCR Methods Appl. 1993 Dec;3(3):186-94 PMID: 8118401
  15. MODELTEST: testing the model of DNA substitution.
    Bioinformatics. 1998;14(9):817-8 PMID: 9918953
  16. Flagellate predation on a bacterial model community: interplay of size-selective grazing, specific bacterial cell size, and bacterial community composition.
    Appl Environ Microbiol. 1999 Nov;65(11):4863-72 PMID: 10543797
  17. Automated approach for ribosomal intergenic spacer analysis of microbial diversity and its application to freshwater bacterial communities.
    Appl Environ Microbiol. 1999 Oct;65(10):4630-6 PMID: 10508099
  18. DNA recovery from soils of diverse composition.
    Appl Environ Microbiol. 1996 Feb;62(2):316-22 PMID: 8593035
  19. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA.
    Appl Environ Microbiol. 1993 Mar;59(3):695-700 PMID: 7683183
  20. Microbial community dynamics in Mediterranean nutrient-enriched seawater mesocosms: changes in the genetic diversity of bacterial populations.
    FEMS Microbiol Ecol. 2001 Jan;34(3):243-253 PMID: 11137604
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.69.4.2253-2268.2003
Email
bcrump@mbl.edu
Published
2003-04-00
Pages
2253-68
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC154827
Subset
IM
Databases
GENBANK
AF534425, AF534426, AF534427, AF534428, AF534429, AF534430, AF534431, AF534432, AF534433, AF534434, AF534435, AF534436, AF534437, AF534438, AF534439, AF534440, AF534441, AF534442, AF534443, AF534444, AF534445, AF534446, AF534447, AF534448, AF534449, AF534450, AF534451, AF534452, AF534453, AF534454, AF534455, AF534456, AF534457, AF534458, AF534459, AF534460, AF534461, AF534462, AF534463, AF534464
External Links
PubMed source DOI: 10.1128/AEM.69.4.2253-2268.2003 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2