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PMID: 12671094 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

SPL8, an SBP-box gene that affects pollen sac development in Arabidopsis.

The Plant cell ·Vol. 15 ·No. 4 ·2003-04-00 ·Pages 1009-19

Unte US, Sorensen AM, Pesaresi P, Gandikota M, Leister D, Saedler H, Huijser P

Abstract

SQUAMOSA PROMOTER BINDING PROTEIN-box genes (SBP-box genes) encode plant-specific proteins that share a highly conserved DNA binding domain, the SBP domain. Although likely to represent transcription factors, little is known about their role in development. In Arabidopsis, SBP-box genes constitute a structurally heterogeneous family of 16 members known as SPL genes. For one of these genes, SPL8, we isolated three independent transposon-tagged mutants, all of which exhibited a strong reduction in fertility. Microscopic analysis revealed that this reduced fertility is attributable primarily to abnormally developed microsporangia, which exhibit premeiotic abortion of the sporocytes. In addition to its role in microsporogenesis, the SPL8 knockout also seems to affect megasporogenesis, trichome formation on sepals, and stamen filament elongation. The SPL8 mutants described help to uncover the roles of SBP-box genes in plant development.

MeSH Terms
Alleles Amino Acid Sequence Arabidopsis/genetics,growth & development Arabidopsis Proteins/genetics,metabolism Base Sequence Cell Surface Extensions/genetics,physiology DNA Transposable Elements/genetics DNA-Binding Proteins/genetics,metabolism Flowers/genetics,growth & development,ultrastructure Gene Expression Regulation, Developmental Gene Expression Regulation, Plant In Situ Hybridization Microscopy, Electron, Scanning Molecular Sequence Data Multigene Family Mutation Phenotype Reproduction Sequence Analysis, DNA Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Transcription Factors/genetics,metabolism
Chemicals
Arabidopsis Proteins DNA Transposable Elements DNA-Binding Proteins SPL8 protein, Arabidopsis Transcription Factors
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Unte Ulrike S
Department of Molecular Plant Genetics, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.
Sorensen Anna-Marie
Pesaresi Paolo
Gandikota Madhuri
Leister Dario
Saedler Heinz
Huijser Peter
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.010678
Published
2003-04-00
Pages
1009-19
Language
English
Region
England
NLM ID
9208688
PMCID
PMC152345
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.010678 PMC Full Text View journal details
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