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PMID: 12651889 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Acetylation regulates subcellular localization of the Wnt signaling nuclear effector POP-1.

Genes & development ·Vol. 17 ·No. 6 ·2003-03-15 ·Pages 717-22

Gay F, Calvo D, Lo MC, Ceron J, Maduro M, Lin R, Shi Y

Abstract

Lymphoid enhancer factor/T-cell factor (LEF/TCF) are transcription factors that mediate the Wnt signaling pathway, and have crucial roles during embryonic development in various organisms. Here we report that acetylation enhances nuclear retention of POP-1, the Caenorhabditis elegans LEF/TCF homolog, through increasing nuclear import and blocking nuclear export. We identify three lysines that are acetylated in vivo, and demonstrate their essential requirement for proper nuclear localization and biological activity of POP-1 during C. elegans embryogenesis. The conservation of these lysines among other LEF/TCF family members suggests that acetylation may be an important, evolutionarily conserved mechanism regulating subcellular distribution of LEF/TCF factors.

MeSH Terms
Acetylation Animals Animals, Genetically Modified Blotting, Western COS Cells Caenorhabditis elegans Caenorhabditis elegans Proteins Cell Nucleus/metabolism DNA-Binding Proteins/metabolism Female Gene Expression Regulation, Developmental Glutathione Transferase/metabolism Green Fluorescent Proteins HeLa Cells High Mobility Group Proteins/metabolism Humans Luminescent Proteins/metabolism Lysine/metabolism Male Mutagenesis, Site-Directed Mutation Nuclear Proteins/metabolism Phosphorylation Precipitin Tests Proto-Oncogene Proteins/metabolism Recombinant Fusion Proteins/metabolism Signal Transduction Trans-Activators/metabolism Wnt Proteins Zebrafish Proteins
Chemicals
Caenorhabditis elegans Proteins DNA-Binding Proteins High Mobility Group Proteins Luminescent Proteins Nuclear Proteins Proto-Oncogene Proteins Recombinant Fusion Proteins Trans-Activators Wnt Proteins Zebrafish Proteins pop-1 protein, C elegans Green Fluorescent Proteins Glutathione Transferase Lysine
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Gay Frédérique
Department of Pathology, Harvard Medical School, Boston, MA 02115, USA.
Calvo Dominica
Lo Miao-Chia
Ceron Julian
Maduro Morris
Lin Rueyling
Shi Yang
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1042403
Published
2003-03-15
Pages
717-22
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC196022
Subset
IM
Grants
NIGMS NIH HHS · R01 GM053874 · United States
NIGMS NIH HHS · R01 GM058012 · United States
NIGMS NIH HHS · GM 53874 · United States
NIGMS NIH HHS · GM 58012 · United States
External Links
PubMed source DOI: 10.1101/gad.1042403 PMC Full Text View journal details
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