Home LiteratureArticle Details
PMID: 1263250 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation of the major intrinsic transmembrane protein of the human erythrocyte membrane.

The Journal of membrane biology ·Vol. 26 ·No. 2-3 ·1976-03-18 ·Pages 173-87

Furthmayr H, Kahane I, Marchesi VT

Abstract

The major intrinsic protein of the human erythrocyte membrane commonly referred to as "Band 3", was isolated by a multi-step procedure. Extraction of ghost membranes in dilute solutions of lithium diiodosalicylate removed most of the proteins considered to be extrinsic to the membrane. The resulting membrane fragments were solubilized in sodium dodecyl sulfate, and the major sialoglycoprotein (glycophorin A) was removed by wheat germ agglutinin-Sepharose affinity chromatography. Gel filtration in sodium dodecyl sulfate was used as the final step to yield the band 3 polypeptide in electrophoretically homogeneous form.

MeSH Terms
Biological Transport, Active Blood Proteins/isolation & purification,metabolism Cell Membrane/metabolism,ultrastructure Erythrocytes/metabolism Humans Microscopy, Electron Salicylates/blood
Chemicals
Blood Proteins Salicylates
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Furthmayr H
Kahane I
Marchesi V T
References (36)
36 references, click to expand
  1. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
    Biochemistry. 1971 Jun 22;10(13):2606-17 PMID: 4326772
  2. Photochemical labeling of the surface proteins of human erythrocytes.
    Biochemistry. 1974 Jun 18;13(13):2720-6 PMID: 4847541
  3. Glycoproteins: isolation from cellmembranes with lithium diiodosalicylate.
    Science. 1971 Dec 17;174(4015):1247-8 PMID: 5133448
  4. Amino-acid sequence and oligosaccharide attachment sites of human erythrocyte glycophorin.
    Proc Natl Acad Sci U S A. 1975 Aug;72(8):2964-8 PMID: 1059087
  5. The fluid mosaic model of the structure of cell membranes.
    Science. 1972 Feb 18;175(4023):720-31 PMID: 4333397
  6. Selective solubilization of proteins and phospholipids from red blood cell membranes by nonionic detergents.
    J Supramol Struct. 1973;1(3):233-48 PMID: 4804838
  7. The structure of erythrocyte membranes studied by freeze-etching. II. Localization of receptors for phytohemagglutinin and influenza virus to the intramembranous particles.
    J Exp Med. 1972 Jun 1;135(6):1209-27 PMID: 5025437
  8. Binding of cytochalasin B to a red cell membrane protein.
    Biochem Biophys Res Commun. 1974 Dec 23;61(4):1471-6 PMID: 4455264
  9. Asymmetric iodination of the human erythrocyte membrane.
    Biochem Biophys Res Commun. 1973 Sep 18;54(2):494-500 PMID: 4756782
  10. Fractionation of the major sialoglycopeptides of the human red blood cell membrane.
    Biochem Biophys Res Commun. 1975 Jul 8;65(1):113-21 PMID: 1147974
  11. An approach to nearest neighbor analysis of membrane proteins. Application to the human erythrocyte membrane of a method employing cleavable cross-linkages.
    J Biol Chem. 1974 Dec 25;249(24):8005-18 PMID: 4430682
  12. Amidination of the outer and inner surfaces of the human erythrocyte membrane.
    J Mol Biol. 1974 Aug 15;87(3):541-61 PMID: 4444035
  13. [19] Gas-liquid chromatographic analysis of constituent carbohydrates in glycoproteins.
    Methods Enzymol. 1972;25:244-9 PMID: 23014406
  14. External labeling of cell surface galactose and galactosamine in glycolipid and glycoprotein of human erythrocytes.
    J Biol Chem. 1973 Jun 25;248(12):4311-7 PMID: 4711609
  15. A major protein which spans the human erythrocyte membrane.
    J Mol Biol. 1971 Jul 28;59(2):351-7 PMID: 5566420
  16. Subunit structure of human erythrocyte glycophorin A.
    Biochemistry. 1976 Mar 9;15(5):1137-44 PMID: 175830
  17. Major glycoprotein of the human erythrocyte membrane: evidence for an amphipathic molecular structure.
    Arch Biochem Biophys. 1973 Mar;155(1):167-83 PMID: 4712445
  18. The transmembrane proteins in the plasma membrane of normal human erythrocytes.
    J Biol Chem. 1974 Dec 10;249(23):7568-73 PMID: 4436325
  19. Lactoperoxidase labeling of erythrocyte membranes from the inside and outside.
    Biochim Biophys Acta. 1974 Apr 29;345(2):141-53 PMID: 4407521
  20. N-terminal amino acid analysis reveal peptide heterogeneity in major electrophoretic protein components of erythrocyte ghosts.
    Biochim Biophys Acta. 1973 Dec 22;330(3):356-61 PMID: 4772287
  21. Isolation of membrane glycoproteins by affinity chromatography in the presence of detergents.
    Biochim Biophys Acta. 1976 Mar 19;426(3):464-76 PMID: 1268207
  22. Cell surface labeling of erythrocyte glycoproteins by galactose oxidase and Mn++-catalyzed coupling reaction with methionine sulfone hydrazide.
    Biochem Biophys Res Commun. 1975 Jan 2;64(3):1028-35 PMID: 1147956
  23. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.
    J Cell Biol. 1963 Apr;17:208-12 PMID: 13986422
  24. The enzymatic iodination of the red cell membrane.
    J Cell Biol. 1972 Nov;55(2):390-405 PMID: 5076780
  25. The receptor proteins for concanavalin A and Lens culinaris phytohemagglutinin in the membrane of the human erythrocyte.
    J Biol Chem. 1974 Jul 25;249(14):4398-403 PMID: 4211043
  26. Major human erythrocyte glycoprotein spans the cell membrane.
    Nat New Biol. 1971 Jun 23;231(25):229-32 PMID: 5284359
  27. Modification and introduction of a specific radioactive label into the erythrocyte membrane sialoglycoproteins.
    Biochem Biophys Res Commun. 1972 Jul 11;48(1):242-51 PMID: 4339463
  28. Separation and some properties of the major proteins of the human erythrocyte membrane.
    Biochem J. 1972 Sep;129(2):333-47 PMID: 4643321
  29. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  30. Topographical distribution of complex carbohydrates in the erythrocyte membrane.
    J Biol Chem. 1974 Apr 10;249(7):2135-42 PMID: 4818830
  31. A pyrrolidonecarboxylate peptidase from the particulate fraction of Klebsiella cloacae. Purification of the stable enzyme and its use in releasing the NH2 terminus from pyrrolidonecarboxylyl peptides and proteins.
    J Biol Chem. 1974 Dec 25;249(24):7729-36 PMID: 4430671
  32. Selective solubilization of proteins from red blood cell membranes by protein perturbants.
    J Supramol Struct. 1973;1(3):220-32 PMID: 4804837
  33. A membrane protein from human erythrocytes involved in anion exchange.
    J Biol Chem. 1975 Jan 25;250(2):675-83 PMID: 1112782
  34. Membrane proteins related to anion permeability of human red blood cells. I. Localization of disulfonic stilbene binding sites in proteins involved in permeation.
    J Membr Biol. 1974;15(3):207-26 PMID: 4838037
  35. The thiobarbituric acid assay of sialic acids.
    J Biol Chem. 1959 Aug;234(8):1971-5 PMID: 13672998
  36. Cross-linking the major proteins of the isolated erythrocyte membrane.
    J Mol Biol. 1972 May 14;66(2):295-305 PMID: 5038456
Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01868872
Published
1976-03-18
Pages
173-87
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01868872 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2