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PMID: 12629067 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Preferential accumulation of antigen-specific effector CD4 T cells at an antigen injection site involves CD62E-dependent migration but not local proliferation.

The Journal of experimental medicine ·Vol. 197 ·No. 6 ·2003-03-17 ·Pages 751-62

Reinhardt RL, Bullard DC, Weaver CT, Jenkins MK

Abstract

The migration of antigen-specific T cells to nonlymphoid tissues is thought to be important for the elimination of foreign antigens from the body. However, recent results showing the migration of activated T cells into many nonlymphoid tissues raised the possibility that antigen-specific T cells do not migrate preferentially to nonlymphoid tissues containing antigen. We addressed this question by tracking antigen-specific CD4 T cells in the whole body after a localized subcutaneous antigen injection. Antigen-specific CD4 T cells proliferated in the skin-draining lymph nodes and the cells that underwent the most cell divisions acquired the ability to bind to CD62P. As time passed, CD62P-binding antigen-specific CD4 T cells with interferon gamma production potential accumulated preferentially at the site of antigen injection but only in recipients that expressed CD62E. Surprisingly, these T cells did not proliferate in the injection site despite showing evidence of more cell divisions than the T cells in the draining lymph nodes. The results suggest that the most divided effector CD4 T cells from the lymph nodes enter the site of antigen deposition via recognition of CD62E on blood vessels and are retained there in a nonproliferative state via recognition of peptide-major histocompatibility complex II molecules.

MeSH Terms
Adoptive Transfer Animals Antigens/immunology Antimetabolites/metabolism Bromodeoxyuridine/metabolism CD4-Positive T-Lymphocytes/immunology,physiology Cell Division/physiology Cell Movement/physiology E-Selectin/metabolism Immunization Immunohistochemistry Interferon-gamma/metabolism Interleukin-2/metabolism Mice Mice, Inbred Strains Ovalbumin/administration & dosage,immunology Protein Binding
Chemicals
Antigens Antimetabolites E-Selectin Interleukin-2 Interferon-gamma Ovalbumin Bromodeoxyuridine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Reinhardt R Lee
Department of Microbiology and the Center for Immunology, University of Minnesota, Minneapolis, MN 55455, USA.
Bullard Daniel C
Weaver Casey T
Jenkins Marc K
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.20021690
Published
2003-03-17
Epub
2003-00-10
Pages
751-62
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2193845
Subset
IM
Grants
NIAMS NIH HHS · AR46404 · United States
NIAID NIH HHS · AI35296 · United States
NIAID NIH HHS · R01 AI035783 · United States
NIAID NIH HHS · T32 AI007313 · United States
NIAID NIH HHS · AI13578 · United States
NIAID NIH HHS · P01 AI035296 · United States
NIAID NIH HHS · R01 AI039614 · United States
NIAID NIH HHS · R37 AI027998 · United States
NIAID NIH HHS · R21 AI035783 · United States
NIAID NIH HHS · R01 AI027998 · United States
NIAID NIH HHS · AI27998 · United States
NIAID NIH HHS · AI39614 · United States
External Links
PubMed source DOI: 10.1084/jem.20021690 PMC Full Text View journal details
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