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PMID: 12628172 Published · ppublish English Journal Article

Opioid-induced quantal slowing reveals dual networks for respiratory rhythm generation.

Neuron ·Vol. 37 ·No. 5 ·2003-03-06 ·Pages 821-6

Mellen NM, Janczewski WA, Bocchiaro CM, Feldman JL

Abstract

Current consensus holds that a single medullary network generates respiratory rhythm in mammals. Pre-Bötzinger Complex inspiratory (I) neurons, isolated in transverse slices, and preinspiratory (pre-I) neurons, found only in more intact en bloc preparations and in vivo, are each proposed as necessary for rhythm generation. Opioids slow I, but not pre-I, neuronal burst periods. In slices, opioids gradually lengthened respiratory periods, whereas in more intact preparations, periods jumped nondeterministically to integer multiples of the control period (quantal slowing). These findings suggest that opioid-induced quantal slowing results from transmission failure of rhythmic drive from pre-I neurons to preBötC I networks, depressed below threshold for spontaneous rhythmic activity. Thus, both I (in the slice), and pre-I neurons are sufficient for respiratory rhythmogenesis.

MeSH Terms
Action Potentials/drug effects,physiology Animals Biological Clocks/drug effects,physiology Brain Stem/drug effects,physiology Enkephalin, Ala(2)-MePhe(4)-Gly(5)-/pharmacology In Vitro Techniques Narcotics/pharmacology Nerve Net/drug effects,physiology Rats Receptors, Opioid, mu/agonists,physiology Respiration/drug effects
Chemicals
Narcotics Receptors, Opioid, mu Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Mellen Nicholas M
Department of Neurobiology, University of California, Los Angeles, P.O. Box 951763, Los Angeles, CA 90095, USA. nmellen@ucla.edu
Janczewski Wiktor A
Bocchiaro Christopher M
Feldman Jack L
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Article Info
Journal
Neuron
Abbr.
Neuron
ISSN
0896-6273
DOI
10.1016/s0896-6273(03)00092-8
Email
nmellen@ucla.edu
Published
2003-03-06
Pages
821-6
Language
English
Region
United States
NLM ID
8809320
PMCID
PMC3210017
Subset
IM
Grants
NHLBI NIH HHS · R01 HL040959 · United States
NHLBI NIH HHS · R01 HL040959-12 · United States
NHLBI NIH HHS · R01 HL070029 · United States
NHLBI NIH HHS · R01 HL070029-02 · United States
External Links
PubMed source DOI: 10.1016/s0896-6273(03)00092-8 PMC Full Text View journal details
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