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PMID: 12615908 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations.

The Journal of cell biology ·Vol. 160 ·No. 5 ·2003-03-03 ·Pages 629-33

Sekar RB, Periasamy A

Abstract

The current advances in fluorescence microscopy, coupled with the development of new fluorescent probes, make fluorescence resonance energy transfer (FRET) a powerful technique for studying molecular interactions inside living cells with improved spatial (angstrom) and temporal (nanosecond) resolution, distance range, and sensitivity and a broader range of biological applications.

MeSH Terms
Animals Eukaryotic Cells/cytology,metabolism Fluorescence Resonance Energy Transfer/methods,trends Fluorescent Dyes Green Fluorescent Proteins Humans Image Processing, Computer-Assisted/methods,trends Luminescent Proteins Microscopy, Fluorescence/methods,trends Proteins/metabolism
Chemicals
Fluorescent Dyes Luminescent Proteins Proteins Green Fluorescent Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sekar Rajesh Babu
W.M. Keck Center for Cellular Imaging, Department of Biology, Gilmer Hall (064), University of Virginia, Charlottesville, VA 22904, USA.
Periasamy Ammasi
References (23)
23 references, click to expand
  1. Genetically encoded fluorescent reporters of protein tyrosine kinase activities in living cells.
    Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15003-8 PMID: 11752449
  2. Localized Rac activation dynamics visualized in living cells.
    Science. 2000 Oct 13;290(5490):333-7 PMID: 11030651
  3. GFP imaging: methodology and application to investigate cellular compartmentation in plants.
    J Exp Bot. 2001 Apr;52(356):529-39 PMID: 11373302
  4. Fluorescence ratio imaging of cyclic AMP in single cells.
    Nature. 1991 Feb 21;349(6311):694-7 PMID: 1847505
  5. Diheteroarylethenes as thermally stable photoswitchable acceptors in photochromic fluorescence resonance energy transfer (pcFRET).
    J Am Chem Soc. 2002 Jun 26;124(25):7481-9 PMID: 12071757
  6. Diffusional mobility of Golgi proteins in membranes of living cells.
    Science. 1996 Aug 9;273(5276):797-801 PMID: 8670420
  7. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer.
    Curr Biol. 1996 Feb 1;6(2):178-82 PMID: 8673464
  8. Characterization of one- and two-photon excitation fluorescence resonance energy transfer microscopy.
    Methods. 2003 Jan;29(1):58-73 PMID: 12543072
  9. The use of FRET imaging microscopy to detect protein-protein interactions and protein conformational changes in vivo.
    Curr Opin Struct Biol. 2001 Oct;11(5):573-8 PMID: 11785758
  10. Reliable and global measurement of fluorescence resonance energy transfer using fluorescence microscopes.
    Biophys J. 2001 Oct;81(4):2395-402 PMID: 11566809
  11. Use of resonance energy transfer to monitor membrane fusion.
    Biochemistry. 1981 Jul 7;20(14):4093-9 PMID: 7284312
  12. Fluorescence resonance energy transfer measurements of distances in actin and myosin. A critical evaluation.
    J Muscle Res Cell Motil. 1987 Apr;8(2):97-117 PMID: 3298315
  13. Fluorescence energy transfer detection as a homogeneous DNA diagnostic method.
    Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10756-61 PMID: 9380706
  14. Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin.
    Nature. 1997 Aug 28;388(6645):882-7 PMID: 9278050
  15. Visualization of Pit-1 transcription factor interactions in the living cell nucleus by fluorescence resonance energy transfer microscopy.
    Mol Endocrinol. 1998 Sep;12(9):1410-9 PMID: 9731708
  16. Nanosecond fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy to localize the protein interactions in a single living cell.
    J Microsc. 2002 Jan;205(Pt 1):3-14 PMID: 11856376
  17. Integrins regulate GTP-Rac localized effector interactions through dissociation of Rho-GDI.
    Nat Cell Biol. 2002 Mar;4(3):232-9 PMID: 11862216
  18. Oligomerization of epidermal growth factor receptors on A431 cells studied by time-resolved fluorescence imaging microscopy. A stereochemical model for tyrosine kinase receptor activation.
    J Cell Biol. 1995 Jun;129(6):1543-58 PMID: 7790353
  19. Using GFP in FRET-based applications.
    Trends Cell Biol. 1999 Feb;9(2):57-60 PMID: 10087619
  20. Imaging into the future: visualizing gene expression and protein interactions with fluorescent proteins.
    Nat Cell Biol. 2002 Jan;4(1):E15-20 PMID: 11780139
  21. Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell.
    Trends Cell Biol. 1999 Feb;9(2):48-52 PMID: 10087617
  22. Quantitative fluorescence resonance energy transfer measurements using fluorescence microscopy.
    Biophys J. 1998 May;74(5):2702-13 PMID: 9591694
  23. FRET-based in vivo Ca2+ imaging by a new calmodulin-GFP fusion molecule.
    Nat Struct Biol. 2001 Dec;8(12):1069-73 PMID: 11702071
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200210140
Published
2003-03-03
Pages
629-33
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2173363
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200210140 PMC Full Text View journal details
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