Identification of genes that protect the C. elegans genome against mutations by genome-wide RNAi.

Genes & development ·Vol. 17 ·No. 4 ·2003-02-15 ·Pages 443-8

Pothof J, van Haaften G, Thijssen K, Kamath RS, Fraser AG, Ahringer J, Plasterk RH, Tijsterman M

Abstract

An RNA interference (RNAi)-based genome-wide screen was performed to detect genes that contribute to genome stability in somatic cells of Caenorhabditis elegans. We identified 61 such genes; these also affect spontaneous mutagenesis in the germ line. Their sequence suggests a role in DNA repair and/or replication, in chromatin remodeling, or in cell cycle control; there are also many novel genes that are highly conserved from yeast to human. Because known mutator genes are causally involved in many hereditary and sporadic human cancers, it is likely that some of these new mutators are equally relevant in cancer etiology.

MeSH Terms

Animals Animals, Genetically Modified Caenorhabditis elegans/genetics Caenorhabditis elegans Proteins/classification,genetics Cell Cycle/genetics Chromatin/genetics,metabolism DNA Repair/genetics DNA Replication/genetics Genes, Helminth Genome Helminth Proteins/genetics Membrane Proteins Muscle Proteins/genetics Mutation RNA Interference RNA, Helminth Sequence Homology, Nucleic Acid

Chemicals

Caenorhabditis elegans Proteins Chromatin Helminth Proteins Membrane Proteins Muscle Proteins RNA, Helminth unc-93 protein, C elegans

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Pothof Joris

Hubrecht Laboratory, Center for Biomedical Genetics, 3584 CT, Utrecht, The Netherlands.

van Haaften Gijs

Thijssen Karen

Kamath Ravi S

Fraser Andrew G

Ahringer Julie

Plasterk Ronald H A

Tijsterman Marcel

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Article Info

Journal

Genes & development

Abbr.

Genes Dev

ISSN

0890-9369

DOI

10.1101/gad.1060703

Published

2003-02-15

Pages

443-8

Language

English

Region

United States

NLM ID

8711660

PMCID

PMC195995

Subset

Grants

Wellcome Trust · 054523 · United Kingdom

External Links

PubMed source DOI: 10.1101/gad.1060703 PMC Full Text View journal details

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Identification of genes that protect the C. elegans genome against mutations by genome-wide RNAi.

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