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题目:: [Establishment of a Human Umbilical Vein Endothelial Cell Line Supporting Hematopoiesis In Vitro]
作者:: Yuan(Hong-Feng),Guo(Zi-Kuan),Liu(Xiao-Dan),Zhang(Shuang-Xi),Mao(Ning)
状态:: 发布时间2003-02-11 , 更新时间 2016-10-18
期刊:: Zhongguo Shi Yan Xue Ye Xue Za Zhi
摘要:: Endothelial cells are the critical cell component of hematopoietic microenvironment. For the purpose of facilitating the study on modulating effect of endothelial cells in hematopoiesis, a human umbilical vein cell line, IEC, was established. Primary human umbilical vein endothelial cells were transfected with the plasmid pSV(3neo) carried SV40 large T antigen by lipofection. The IEC cells expressed factor VIII and the UEA I-binding ratio was about 97%. The chromosome variation was existed in the cell line, with the karyotype 45, XX, -18, 18q(+). The cell line retained the proliferative capacity at least 25 passages. IEC cells stimulated the growth of granulocyte-macrophage progenitor cells in coculture of cord blood CD34(+) cells with IEC cells. It is concluded that IEC cell line possessed the biological features of endothelial cell and supported hematopoiesis in vitro.
语言:: chi
DOI:

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