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PMID: 12562796 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Pyoverdine-mediated regulation of FpvA synthesis in Pseudomonas aeruginosa: involvement of a probable extracytoplasmic-function sigma factor, FpvI.

Journal of bacteriology ·Vol. 185 ·No. 4 ·2003-02-00 ·Pages 1261-5

Rédly GA, Poole K

Abstract

A search of the pvd pyoverdine biosynthesis locus of Pseudomonas aeruginosa identified an open reading frame, PA2387, whose product exhibited a sequence similar to those of a number of so-called extracytoplasmic- function sigma factors responsible for siderophore-dependent expression of iron-siderophore receptors in Escherichia coli and Pseudomonas putida. Deletion of this gene, dubbed fpvI, compromised pyoverdine-dependent FpvA ferric pyoverdine receptor production and fpvA gene expression, while the cloned gene stimulated fpvA expression. A Fur-binding site was identified immediately upstream of fpvI, consistent with the observed iron-regulated expression of fpvI and fpvA.

MeSH Terms
Bacterial Outer Membrane Proteins/biosynthesis,genetics Bacterial Proteins/chemistry,genetics,metabolism Base Sequence Gene Expression Regulation, Bacterial Iron/metabolism Molecular Sequence Data Oligopeptides Pigments, Biological/metabolism Pseudomonas aeruginosa/genetics,metabolism Sigma Factor/chemistry,genetics,metabolism
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins FpvA protein, Pseudomonas aeruginosa Oligopeptides Pigments, Biological Sigma Factor pyoverdin Iron
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rédly Gyula Alan
Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N6.
Poole Keith
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.185.4.1261-1265.2003
Published
2003-02-00
Pages
1261-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC142879
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.185.4.1261-1265.2003 PMC Full Text View journal details
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