Production of trans-lentiviral vector with predictable safety.

Animals Cell Line DNA, Viral Fusion Proteins, gag-pol/genetics,metabolism Gene Products, env/genetics Gene Transfer Techniques Genetic Therapy Genetic Vectors Humans Lentivirus/genetics,physiology Plasmids/genetics Protein Synthesis Inhibitors/metabolism Puromycin/metabolism Quality Control Recombination, Genetic Retroviridae Proteins/genetics,metabolism Transfection Transgenes Virus Assembly Virus Integration Virus Replication

DNA, Viral Fusion Proteins, gag-pol Gene Products, env Protein Synthesis Inhibitors Retroviridae Proteins Puromycin