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PMID: 12525699 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Unique inflammatory RNA profiles of microglia in Creutzfeldt-Jakob disease.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 100 ·No. 2 ·2003-01-21 ·Pages 675-9

Baker CA, Manuelidis L

Abstract

Previous studies in Creutzfeldt-Jakob disease (CJD) have shown that myeloid cells in the periphery as well as derivative microglial cells in the brain are infectious. Microglia can show an activated phenotype before prion protein (PrP) pathology is detectable in brain, and isolated infectious microglia contain very little PrP. To find whether a set of inflammatory genes are significantly induced or suppressed with infection, we analyzed RNA from isolated microglia with relevant cDNA arrays, and identified approximately 30 transcripts not previously examined in any transmissible spongiform encephalopathy. This CJD expression profile contrasted with that of uninfected microglia exposed to prototypic inflammatory stimuli such as lipopolysaccharide and IFN-gamma, as well as PrP amyloid. These findings underscore inflammatory pathways evoked by the infectious agent in brain. Transcript profiles unique for CJD microglia and other myeloid cells provide opportunities for more sensitive preclinical diagnoses of infectious and noninfectious neurodegenerative diseases.

MeSH Terms
Animals Cells, Cultured Creutzfeldt-Jakob Syndrome/metabolism Gene Expression Profiling Inflammation/metabolism Interferon-gamma/pharmacology Lipopolysaccharides/pharmacology Mice Microglia/metabolism Oligonucleotide Array Sequence Analysis PrPSc Proteins/pharmacology Reverse Transcriptase Polymerase Chain Reaction
Chemicals
Lipopolysaccharides PrPSc Proteins Interferon-gamma
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Baker Christopher A
Section of Neuropathology, Yale University School of Medicine, 333 Cedar Street, FMB 11, New Haven, CT 06510, USA.
Manuelidis Laura
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0237313100
Published
2003-01-21
Epub
2003-00-13
Pages
675-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC141055
Subset
IM
Grants
NINDS NIH HHS · R01 NS012674 · United States
NINDS NIH HHS · R56 NS012674 · United States
NINDS NIH HHS · NS 012674 · United States
NINDS NIH HHS · NS 034569 · United States
External Links
PubMed source DOI: 10.1073/pnas.0237313100 PMC Full Text View journal details
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