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PMID: 12522259 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Assembly of single chromatin fibers depends on the tension in the DNA molecule: magnetic tweezers study.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 100 ·No. 2 ·2003-01-21 ·Pages 495-500

Leuba SH, Karymov MA, Tomschik M, Ramjit R, Smith P, Zlatanova J

Abstract

We have used magnetic tweezers to study in real time chaperone-mediated chromatin assembly/disassembly at the level of single chromatin fibers. We find a strong dependence of the rate of assembly on the exerted force, with strong inhibition of assembly at forces exceeding 10 pN. During assembly, and especially at higher forces, occasional abrupt increases in the length of the fiber were observed, giving a clear indication of reversibility of the assembly process. This result is a clear demonstration of the dynamic equilibrium between nucleosome assembly and disassembly at the single chromatin fiber level.

MeSH Terms
Chromatin/chemistry DNA/chemistry Magnetics Microscopy, Atomic Force Nucleosomes/chemistry
Chemicals
Chromatin Nucleosomes DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Leuba Sanford H
Department of Cell Biology and Physiology, School of Medicine, Hillman Cancer Center, University of Pittsburgh Cancer Institute Research Pavilion, Pittsburgh, PA 15213, USA. leuba@pitt.edu
Karymov Mikhail A
Tomschik Miroslav
Ramjit Ravi
Smith Paul
Zlatanova Jordanka
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0136890100
Email
leuba@pitt.edu
Published
2003-01-21
Epub
2003-00-09
Pages
495-500
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC141023
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.0136890100 PMC Full Text View journal details
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