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PMID: 12522149 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Impaired B cell development and function in mice with a targeted disruption of the homeobox gene Hex.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 100 ·No. 2 ·2003-01-21 ·Pages 556-61

Bogue CW, Zhang PX, McGrath J, Jacobs HC, Fuleihan RL

Abstract

Hex is a homeobox gene that is expressed in all stages of B cell development except plasma cells. We studied lymphocyte development in the absence of Hex by using the RAG1-deficient blastocyst complementation system because homozygous disruption of Hex is embryonic lethal. Hex(-/-);RAG1(-/-) chimeric mice had severely reduced numbers of mature B cells, pre-B cells, and CD5(+) B cells with a striking 15-fold increase in the percentage of B220(-)CD19(+) cells in the bone marrow. Hex(-/-);RAG1(-/-) chimeric mice failed to generate IgG antibodies to T cell-independent antigens, although their serum IgM levels and antibody responses to T cell-dependent antigens were intact. Therefore, Hex is necessary for B cell development and function and its absence results in a dramatic increase in B220(-)CD19(+) cells.

MeSH Terms

Animals Antigens, CD19/analysis B-Lymphocytes/physiology Genes, Homeobox/physiology Homeodomain Proteins/genetics,physiology Immunoglobulin G/biosynthesis Immunoglobulin M/biosynthesis Leukocyte Common Antigens/analysis Lymphopoiesis Mice Mice, Inbred C57BL Transcription Factors

Chemicals

Antigens, CD19 Hhex protein, mouse Homeodomain Proteins Immunoglobulin G Immunoglobulin M Transcription Factors RAG-1 protein Leukocyte Common Antigens

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Bogue Clifford W

Yale Child Health Research Center, Department of Pediatrics, Yale University School of Medicine, New Haven, CT 06519, USA. clifford.bogue@yale.edu

Zhang Ping-Xia

McGrath James

Jacobs Harris C

Fuleihan Ramsay L

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21 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.0236979100

clifford.bogue@yale.edu

Published

2003-01-21

Epub

2003-00-08

Pages

556-61

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC141034

Subset

Grants

NHLBI NIH HHS · K08 HL 03471 · United States

External Links

PubMed source DOI: 10.1073/pnas.0236979100 PMC Full Text View journal details

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Impaired B cell development and function in mice with a targeted disruption of the homeobox gene Hex.

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