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PMID: 12499164 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Contributions of MexAB-OprM and an EmrE homolog to intrinsic resistance of Pseudomonas aeruginosa to aminoglycosides and dyes.

Antimicrobial agents and chemotherapy ·Vol. 47 ·No. 1 ·2003-01-00 ·Pages 27-33

Li XZ, Poole K, Nikaido H

Abstract

Of the six putative small multidrug resistance (SMR) family proteins of Pseudomonas aeruginosa, a protein encoded by the PA4990 gene (emrE(Pae)) shows the highest identity to the well-characterized EmrE efflux transporter of Escherichia coli. Reverse transcription-PCR confirmed the expression of emrE(Pae) in the wild-type strain of P. aeruginosa. Using isogenic emrE(Pae), mexAB-oprM, and/or mexB deletion mutants, the contributions of the EmrE protein and the MexAB-OprM efflux system to drug resistance in P. aeruginosa were assessed by a drug susceptibility test carried out in a low-ionic-strength medium, Difco nutrient broth. We found that EmrE(Pae) contributed to intrinsic resistance not only to ethidium bromide and acriflavine but also to aminoglycosides. In this low-ionic-strength medium, MexAB-OprM was also shown to contribute to aminoglycoside resistance, presumably via active efflux. Aminoglycoside resistance caused by these two pumps could not be demonstrated in high-ionic-strength media, such as Luria broth or Mueller-Hinton broth. The EmrE-dependent efflux of ethidium bromide was confirmed by a continuous fluorescence assay.

MeSH Terms
Amino Acid Sequence Aminoglycosides Anti-Bacterial Agents/pharmacology Antiporters/drug effects,genetics Bacterial Outer Membrane Proteins/drug effects,genetics Carrier Proteins/drug effects,genetics Drug Resistance, Bacterial/genetics Escherichia coli Proteins Membrane Proteins/drug effects,genetics Membrane Transport Proteins Microbial Sensitivity Tests Plasmids Pseudomonas aeruginosa/drug effects,genetics
Chemicals
Aminoglycosides Anti-Bacterial Agents Antiporters Bacterial Outer Membrane Proteins Carrier Proteins Escherichia coli Proteins Membrane Proteins Membrane Transport Proteins MexA protein, Pseudomonas aeruginosa EmrE protein, E coli
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Li Xian-Zhi
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3206, USA.
Poole Keith
Nikaido Hiroshi
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31 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.47.1.27-33.2003
Published
2003-01-00
Pages
27-33
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC149025
Subset
IM
Grants
NIAID NIH HHS · R01 AI009644 · United States
NIAID NIH HHS · R37 AI009644 · United States
NIAID NIH HHS · AI-09644 · United States
External Links
PubMed source DOI: 10.1128/AAC.47.1.27-33.2003 PMC Full Text View journal details
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