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PMID: 12473838 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

HIV type 1 group M clades infecting subjects from rural villages in equatorial rain forests of Cameroon.

Journal of acquired immune deficiency syndromes (1999) ·Vol. 31 ·No. 5 ·2002-12-15 ·Pages 495-505

Zhong P, Burda S, Urbanski M, Kenfack H, Tongo M, Heyndrickx L, Nanfack A, Shang J, Agyingi L, Zolla-Pazner S, Zekeng L, Nyambi P

Abstract

Though the HIV-1 subtypes infecting patients living in urban and semi-urban areas in Cameroon have been reported, information on the subtypes infecting patients in rural villages is lacking. To begin to understand the diversity of the HIV-1 group M subtypes infecting persons living in rural villages in the equatorial rain forest regions of Cameroon, 49 plasma samples from 14 rural villages in four provinces of Cameroon were analyzed using heteroduplex mobility analysis (HMA), DNA sequencing, and phylogenetic tree analysis on the basis of env C2V5, gag, or pol regions. Sixty-one percent of the group M infections were clade A or CRF02_AG-like as subtyped by env and gag. Of the remaining group M infections, 12% were either A or CRF02_AG-like or CRF01_AE-like in recombination with other clades; 25% were infections that were entirely non-A or non-CRF02_AG-like; and 2% were CRF11_cpx. The HIV-1 group M clades identified included A, D, F (F2), G, and H. The CRF strains identified were CRF02_AG-like, CRF01_AE-like, and CRF11_cpx. Two new intersubtype recombinant infections, H/G and A/F2, were identified. This study suggests that the HIV-1 diversity in rural villages in the equatorial rain forest of Cameroon is at least as broad as has been observed in major cities of Cameroon and that multiple HIV-1 group M subtypes are infecting persons living in the countryside of Cameroon.

MeSH Terms

Adolescent Adult Amino Acid Sequence Cameroon/epidemiology Evolution, Molecular Female Genes, Viral/genetics HIV Infections/epidemiology,virology HIV-1/classification,genetics,isolation & purification Heteroduplex Analysis Humans Male Middle Aged Molecular Sequence Data Phylogeny RNA, Viral/genetics Reverse Transcriptase Polymerase Chain Reaction Rural Health

Chemicals

RNA, Viral

Authors & Affiliations

12 authors, click to expand affiliations / ORCID

Zhong Ping

Department of Pathology, New York University School of Medicine, New York, NY, USA.

Burda Sherri

Urbanski Mateusz

Kenfack Henriette

Tongo Marcel

Heyndrickx Leo

Nanfack Aubin

Shang Judith

Agyingi Lucy

Zolla-Pazner Susan

Zekeng Leopold

Nyambi Phillipe

Article Info

Journal

Journal of acquired immune deficiency syndromes (1999)

Abbr.

J Acquir Immune Defic Syndr

ISSN

1525-4135

DOI

10.1097/00126334-200212150-00007

Published

2002-12-15

Pages

495-505

Language

English

Region

United States

NLM ID

100892005

Subset

Grants

NIAID NIH HHS · AI 27742 · United States

NIAID NIH HHS · AI 47053 · United States

FIC NIH HHS · TW 01409 · United States

Databases

GENBANK

AF508796, AF509506, AF509507, AF509508, AF509509, AF509510, AF509511, AF509512, AF509513, AF509514, AF509515, AF509516, AF509517, AF509518, AF509519, AF509520, AF509521, AF509522, AF509523, AF509524, AF509525, AF509526, AF509527, AF509528, AF511011, AF511012, AF511013, AF511014, AF511015, AF511016, AF511017, AF511018, AF511019, AF511020, AF511021, AF511022, AF511023, AF511024, AF511025, AF511026, AF511027, AF511028, AF511029, AF511030, AF511031, AF511032, AF511033, AF511034, AF511035, AF511036

External Links

PubMed source DOI: 10.1097/00126334-200212150-00007 View journal details

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HIV type 1 group M clades infecting subjects from rural villages in equatorial rain forests of Cameroon.

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