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PMID: 12460189 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Down-regulation of the myeloid homeobox protein Hex is essential for normal T-cell development.

Immunology ·Vol. 107 ·No. 4 ·2002-12-00 ·Pages 444-51

Mack DL, Leibowitz DS, Cooper S, Ramsey H, Broxmeyer HE, Hromas R

Abstract

The haematopoietic homeobox gene Hex (also called Prh) is expressed in myeloid cells and B cells but not T cells. To investigate whether Hex levels might play a role in myeloid versus T-cell development, two types of transgenic mouse lines were constructed, each with ectopic expression of Hex in T cells (CD11a/Hex and Lck/Hex). Both these types of transgenic mouse had the same defects in T-cell maturation, indicating that proper T-cell development may be dependent not just on the up-regulation of lymphoid-specific transcriptional regulators but also on the co-ordinated down-regulation of myeloid-specific transcriptional regulators such as Hex. In addition, Hex over-expression significantly increased myeloid progenitor cycling, which may explain its role in retrovirally induced murine leukaemia.

MeSH Terms
Animals Cell Line/immunology Down-Regulation/genetics,immunology Flow Cytometry/methods Gene Expression/genetics,immunology Genes, Homeobox/genetics,immunology Genotype Hematopoietic Stem Cells/immunology Homeodomain Proteins/genetics,immunology Lymphopenia/genetics,immunology Mice Mice, Inbred C3H Mice, Transgenic T-Lymphocytes/immunology Thymus Gland/immunology Transcription Factors
Chemicals
Hhex protein, mouse Homeodomain Proteins Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Mack David L
Department of Medicine, the Walther Oncology Center, Indiana University Medical Center, Indianapolis, IN 46202, USA.
Leibowitz David S
Cooper Scott
Ramsey Heather
Broxmeyer Hal E
Hromas Robert
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Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
DOI
10.1046/j.1365-2567.2002.01523.x
Published
2002-12-00
Pages
444-51
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1782816
Subset
IM
Grants
NHLBI NIH HHS · HL48914 · United States
NHLBI NIH HHS · HL 66308 · United States
NIDDK NIH HHS · R01 DK053674 · United States
NIDDK NIH HHS · DK53674 · United States
NHLBI NIH HHS · HL56416 · United States
NHLBI NIH HHS · R01 HL056416 · United States
NHLBI NIH HHS · R01 HL066308 · United States
External Links
PubMed source DOI: 10.1046/j.1365-2567.2002.01523.x PMC Full Text View journal details
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