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PMID: 12455993 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Divergent subunit interactions among fungal mRNA 5'-capping machineries.

Eukaryotic cell ·Vol. 1 ·No. 3 ·2002-06-00 ·Pages 448-57

Takagi T, Cho EJ, Janoo RT, Polodny V, Takase Y, Keogh MC, Woo SA, Fresco-Cohen LD, Hoffman CS, Buratowski S

Abstract

The Saccharomyces cerevisiae mRNA capping enzyme consists of two subunits: an RNA 5'-triphosphatase (RTPase) and GTP::mRNA guanylyltransferase (GTase). The GTase subunit (Ceg1) binds to the phosphorylated carboxyl-terminal domain of the largest subunit (CTD-P) of RNA polymerase II (pol II), coupling capping with transcription. Ceg1 bound to the CTD-P is inactive unless allosterically activated by interaction with the RTPase subunit (Cet1). For purposes of comparison, we characterize here the related GTases and RTPases from the yeasts Schizosaccharomyces pombe and Candida albicans. Surprisingly, the S. pombe capping enzyme subunits do not interact with each other. Both can independently interact with CTD-P of pol II, and the GTase is not repressed by CTD-P binding. The S. pombe RTPase gene (pct1+) is essential for viability. Pct1 can replace the S. cerevisiae RTPase when GTase activity is supplied by the S. pombe or mouse enzymes but not by the S. cerevisiae GTase. The C. albicans capping enzyme subunits do interact with each other. However, this interaction is not essential in vivo. Our results reveal an unexpected diversity among the fungal capping machineries.

MeSH Terms
Acid Anhydride Hydrolases/chemistry,genetics,metabolism Amino Acid Sequence Animals Base Sequence Candida albicans/enzymology,genetics DNA Polymerase II/chemistry,genetics,metabolism DNA, Fungal/genetics DNA-Binding Proteins/chemistry,genetics,metabolism Fungal Proteins/chemistry,genetics,metabolism Fungi/enzymology,genetics Genes, Fungal In Vitro Techniques Mice Molecular Sequence Data Nucleotidyltransferases/chemistry,genetics,metabolism Plasmids/genetics Protein Subunits Saccharomyces cerevisiae/enzymology,genetics Schizosaccharomyces/enzymology,genetics Schizosaccharomyces pombe Proteins Sequence Homology, Amino Acid Species Specificity
Chemicals
DNA, Fungal DNA-Binding Proteins Fungal Proteins Protein Subunits Schizosaccharomyces pombe Proteins res2 protein, S pombe Nucleotidyltransferases mRNA guanylyltransferase DNA Polymerase II Acid Anhydride Hydrolases RNA triphosphatase
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Takagi Toshimitsu
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 021151, USA.
Cho Eun-Jung
Janoo Rozmin T K
Polodny Vladimir
Takase Yasutaka
Keogh Michael C
Woo Sue-Ann
Fresco-Cohen Lucille D
Hoffman Charles S
Buratowski Stephen
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Article Info
Journal
Eukaryotic cell
Abbr.
Eukaryot Cell
ISSN
1535-9778
DOI
10.1128/EC.1.3.448-457.2002
Published
2002-06-00
Pages
448-57
Language
English
Region
United States
NLM ID
101130731
PMCID
PMC118010
Subset
IM
Grants
NIGMS NIH HHS · R01 GM056663 · United States
NIGMS NIH HHS · GM56663 · United States
External Links
PubMed source DOI: 10.1128/EC.1.3.448-457.2002 PMC Full Text View journal details
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