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PMID: 12446745 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The relationship between force and focal complex development.

The Journal of cell biology ·Vol. 159 ·No. 4 ·2002-11-25 ·Pages 695-705

Galbraith CG, Yamada KM, Sheetz MP

Abstract

To adhere and migrate, cells must be capable of applying cytoskeletal force to the extracellular matrix (ECM) through integrin receptors. However, it is unclear if connections between integrins and the ECM are immediately capable of transducing cytoskeletal contraction into migration force, or whether engagement of force transmission requires maturation of the adhesion. Here, we show that initial integrin-ECM adhesions become capable of exerting migration force with the recruitment of vinculin, a marker for focal complexes, which are precursors of focal adhesions. We are able to induce the development of focal complexes by the application of mechanical force to fibronectin receptors from inside or outside the cell, and we are able to extend focal complex formation to vitronectin receptors by the removal of c-Src. These results indicate that cells use mechanical force as a signal to strengthen initial integrin-ECM adhesions into focal complexes and regulate the amount of migration force applied to individual adhesions at localized regions of the advancing lamella.

MeSH Terms
3T3 Cells Animals CSK Tyrosine-Protein Kinase Cell Adhesion/physiology Cell Movement/physiology Concanavalin A/metabolism Cytoskeleton/metabolism Extracellular Matrix/metabolism Fibronectins/metabolism Focal Adhesions/metabolism Integrins/metabolism Ligands Mice Models, Biological Protein-Tyrosine Kinases/metabolism Recombinant Fusion Proteins/metabolism Vinculin/genetics,metabolism Vitronectin/metabolism src-Family Kinases
Chemicals
Fibronectins Integrins Ligands Recombinant Fusion Proteins Vitronectin Concanavalin A Vinculin Protein-Tyrosine Kinases CSK Tyrosine-Protein Kinase src-Family Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Galbraith Catherine G
Duke University Medical Center, Durham, NC 27710, USA.
Yamada Kenneth M
Sheetz Michael P
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200204153
Published
2002-11-25
Pages
695-705
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2173098
Subset
IM
Grants
NIGMS NIH HHS · R01 GM036277 · United States
NIGMS NIH HHS · GM 36277-15 · United States
External Links
PubMed source DOI: 10.1083/jcb.200204153 PMC Full Text View journal details
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