Home LiteratureArticle Details
PMID: 12446563 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

COP9 signalosome components play a role in the mating pheromone response of S. cerevisiae.

EMBO reports ·Vol. 3 ·No. 12 ·2002-12-00 ·Pages 1215-21

Maytal-Kivity V, Piran R, Pick E, Hofmann K, Glickman MH

Abstract

A family of genetically and structurally homologous complexes, the proteasome lid, Cop9 signalosome (CSN) and eukaryotic translation initiation factor 3, mediate different regulatory pathways. The CSN functions in numerous eukaryotes as a regulator of development and signaling, yet until now no evidence for a complex has been found in Saccharomyces cerevisiae. We identified a group of proteins, including a homolog of Csn5/Jab1 and four uncharacterized PCI components, that interact in a manner suggesting they form a complex analogous to the CSN in S. cerevisiae. These newly identified subunits play a role in adaptation to pheromone signaling. Deletants for individual subunits enhance pheromone response and increase mating efficiency. Overexpression of individual subunits or a human homolog mitigates sst2-induced pheromone sensitivity. Csi1, a novel CSN interactor, exhibits opposite phenotypes. Deletants also accumulate Cdc53/cullin in a Rub1-modified form; however, this role of the CSN appears to be distinct from that in the mating pathway.

MeSH Terms
COP9 Signalosome Complex Cell Cycle Proteins/metabolism Cullin Proteins Multiprotein Complexes Peptide Hydrolases Pheromones/metabolism Proteins/metabolism Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins
Chemicals
Cdc53 protein, S cerevisiae Cell Cycle Proteins Csi1 protein, S cerevisiae Cullin Proteins Multiprotein Complexes Pheromones Proteins Saccharomyces cerevisiae Proteins Peptide Hydrolases COP9 Signalosome Complex
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Maytal-Kivity Vered
Department of Biology and Institute for Catalysis Science and Technology, Technion-Israel Institute of Technology, 32000 Haifa, Israel.
Piran Ron
Pick Elah
Hofmann Kay
Glickman Michael H
References (34)
34 references, click to expand
  1. COP9 signalosome-directed c-Jun activation/stabilization is independent of JNK.
    J Biol Chem. 1999 Dec 10;274(50):35297-300 PMID: 10585392
  2. A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae.
    Nature. 2000 Feb 10;403(6770):623-7 PMID: 10688190
  3. Unified nomenclature for the COP9 signalosome and its subunits: an essential regulator of development.
    Trends Genet. 2000 May;16(5):202-3 PMID: 10782111
  4. Electron microscopy and subunit-subunit interaction studies reveal a first architecture of COP9 signalosome.
    J Mol Biol. 2000 Jul 28;300(5):1169-78 PMID: 10903862
  5. Lineage-specific loss and divergence of functionally linked genes in eukaryotes.
    Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11319-24 PMID: 11016957
  6. The subunit 1 of the COP9 signalosome suppresses gene expression through its N-terminal domain and incorporates into the complex through the PCI domain.
    J Mol Biol. 2001 Jan 5;305(1):1-9 PMID: 11114242
  7. Pheromone response, mating and cell biology.
    Curr Opin Microbiol. 2000 Dec;3(6):573-81 PMID: 11121776
  8. Arabidopsis eIF3e (INT-6) associates with both eIF3c and the COP9 signalosome subunit CSN7.
    J Biol Chem. 2001 Jan 5;276(1):334-40 PMID: 11029466
  9. A new hyperrecombination mutation identifies a novel yeast gene, THP1, connecting transcription elongation with mitotic recombination.
    Genetics. 2001 Jan;157(1):79-89 PMID: 11139493
  10. COP9 signalosome-specific phosphorylation targets p53 to degradation by the ubiquitin system.
    EMBO J. 2001 Apr 2;20(7):1630-9 PMID: 11285227
  11. Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome.
    Science. 2001 May 18;292(5520):1382-5 PMID: 11337588
  12. Sensitive protein comparisons with profiles and hidden Markov models.
    Brief Bioinform. 2000 May;1(2):167-78 PMID: 11465028
  13. Saccharomyces cerevisiae protein Pci8p and human protein eIF3e/Int-6 interact with the eIF3 core complex by binding to cognate eIF3b subunits.
    J Biol Chem. 2001 Sep 14;276(37):34948-57 PMID: 11457827
  14. Subunit interaction maps for the regulatory particle of the 26S proteasome and the COP9 signalosome.
    EMBO J. 2001 Dec 17;20(24):7096-107 PMID: 11742986
  15. Functional organization of the yeast proteome by systematic analysis of protein complexes.
    Nature. 2002 Jan 10;415(6868):141-7 PMID: 11805826
  16. G proteins and pheromone signaling.
    Annu Rev Physiol. 2002;64:129-52 PMID: 11826266
  17. Deletion mutants in COP9/signalosome subunits in fission yeast Schizosaccharomyces pombe display distinct phenotypes.
    Mol Biol Cell. 2002 Feb;13(2):493-502 PMID: 11854407
  18. The COP9 signalosome: at the interface between signal transduction and ubiquitin-dependent proteolysis.
    J Cell Sci. 2002 Feb 1;115(Pt 3):467-73 PMID: 11861754
  19. The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction.
    Physiol Rev. 2002 Apr;82(2):373-428 PMID: 11917093
  20. The COP9 signalosome.
    Curr Biol. 2002 Apr 2;12(7):R232 PMID: 11937035
  21. The COP9 signalosome inhibits p27(kip1) degradation and impedes G1-S phase progression via deneddylation of SCF Cul1.
    Curr Biol. 2002 Apr 16;12(8):667-72 PMID: 11967155
  22. Association of the mammalian proto-oncoprotein Int-6 with the three protein complexes eIF3, COP9 signalosome and 26S proteasome.
    FEBS Lett. 2002 Sep 11;527(1-3):15-21 PMID: 12220626
  23. COP9 signalosome subunits 4 and 5 regulate multiple pleiotropic pathways in Drosophila melanogaster.
    Development. 2002 Oct;129(19):4399-409 PMID: 12223399
  24. Role of predicted metalloprotease motif of Jab1/Csn5 in cleavage of Nedd8 from Cul1.
    Science. 2002 Oct 18;298(5593):608-11 PMID: 12183637
  25. The fission yeast COP9/signalosome is involved in cullin modification by ubiquitin-related Ned8p.
    BMC Biochem. 2001;2:7 PMID: 11504566
  26. Conservation of the COP9/signalosome in budding yeast.
    BMC Genet. 2002 Aug 20;3:15 PMID: 12186635
  27. The COP9 complex, a novel multisubunit nuclear regulator involved in light control of a plant developmental switch.
    Cell. 1996 Jul 12;86(1):115-21 PMID: 8689678
  28. Two human cDNAs, including a homolog of Arabidopsis FUS6 (COP11), suppress G-protein- and mitogen-activated protein kinase-mediated signal transduction in yeast and mammalian cells.
    Mol Cell Biol. 1996 Dec;16(12):6698-706 PMID: 8943324
  29. A novel protein complex involved in signal transduction possessing similarities to 26S proteasome subunits.
    FASEB J. 1998 Apr;12(6):469-78 PMID: 9535219
  30. Homologues of 26S proteasome subunits are regulators of transcription and translation.
    Protein Sci. 1998 May;7(5):1250-4 PMID: 9605331
  31. The PCI domain: a common theme in three multiprotein complexes.
    Trends Biochem Sci. 1998 Jun;23(6):204-5 PMID: 9644972
  32. A subcomplex of the proteasome regulatory particle required for ubiquitin-conjugate degradation and related to the COP9-signalosome and eIF3.
    Cell. 1998 Sep 4;94(5):615-23 PMID: 9741626
  33. Arabidopsis homologs of a c-Jun coactivator are present both in monomeric form and in the COP9 complex, and their abundance is differentially affected by the pleiotropic cop/det/fus mutations.
    Plant Cell. 1998 Nov;10(11):1779-90 PMID: 9811788
  34. MPN+, a putative catalytic motif found in a subset of MPN domain proteins from eukaryotes and prokaryotes, is critical for Rpn11 function.
    BMC Biochem. 2002;3:28 PMID: 12370088
Article Info
Journal
EMBO reports
Abbr.
EMBO Rep
ISSN
1469-221X
DOI
10.1093/embo-reports/kvf235
Published
2002-12-00
Epub
2002-00-21
Pages
1215-21
Language
English
Region
England
NLM ID
100963049
PMCID
PMC1308327
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1093/embo-reports/kvf235 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2