Abstract
The recent application of molecular phylogeny to environmental samples has resulted in the discovery of an abundance of unique and previously unrecognized microorganisms. The vast majority of this microbial diversity has proved refractory to cultivation. Here, we describe a universal method that provides access to this immense reservoir of untapped microbial diversity. This technique combines encapsulation of cells in gel microdroplets for massively parallel microbial cultivation under low nutrient flux conditions, followed by flow cytometry to detect microdroplets containing microcolonies. The ability to grow and study previously uncultured organisms in pure culture will enhance our understanding of microbial physiology and metabolic adaptation and will provide new sources of microbial metabolites. We show that this technology can be applied to samples from several different environments, including seawater and soil.
MeSH Terms
Bacteria/classification,genetics,isolation & purification
Bacteriological Techniques
Drug Compounding
Flow Cytometry
Fresh Water/microbiology
Gels
Ghana
Kenya
Particle Size
Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal/genetics
RNA, Ribosomal, 16S/genetics
Seawater/microbiology
Soil Microbiology
Species Specificity
Water Microbiology
Chemicals
Gels
RNA, Ribosomal
RNA, Ribosomal, 16S
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Zengler Karsten
Diversa Corporation, San Diego, CA 92121, USA.
Toledo Gerardo
Rappe Michael
Elkins James
Mathur Eric J
Short Jay M
Keller Martin
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