Control of thymidine kinase synthesis in IHD vaccinia virus-infected thymidine kinase-deficient LM cells.

Journal of virology ·Vol. 16 ·No. 1 ·1975-07-00 ·Pages 210-3

Zaslavsky V, Yakobson E

Abstract

The synthesis of vaccinia virus-induced thymidine kinase is normally arrested several hours after infection. In thymidine kinase-deficient LM cells infected with IHD strain of vaccinia virus, arrest occurs whether or not viral DNA synthesis is inhibited. With virus inactivated by UV irradiation, enzyme synthesis takes place, but arrest is abolished. It is suggested that an early viral genetic function is responsible for the cessation of thymidine kinase synthesis.

MeSH Terms

Animals Cell Line Cell-Free System Connective Tissue Cytarabine/pharmacology DNA, Viral/biosynthesis Dactinomycin/pharmacology Enzyme Induction Floxuridine/pharmacology HeLa Cells Humans Mice Radiation Effects Thymidine Kinase/biosynthesis,metabolism Ultraviolet Rays Vaccinia virus/growth & development,metabolism,radiation effects

Chemicals

DNA, Viral Floxuridine Cytarabine Dactinomycin Thymidine Kinase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Zaslavsky V

Yakobson E

References (12)

12 references, click to expand

Studies on "early" enzymes in HeLa cells infected with vaccinia virus.
Virology. 1965 Sep;27(1):80-93 PMID: 4157610
Vaccinia mRNA synthesis under conditions which prevent uncoating.
Biochem Biophys Res Commun. 1967 Apr 20;27(2):169-75 PMID: 4226809
Viral-induced enzymes and the problem of viral oncogenesis.
Adv Cancer Res. 1968;11:73-221 PMID: 4306735
Electrophoresis of thymidine kinase activity synthesized by cells transformed by herpes simplex virus.
Virology. 1972 Sep;49(3):683-9 PMID: 4342081
Induction of cytoplasmic ribonucleic acid (RNA) synthesis in vaccinia-infected LM cells during inhibition of protein synthesis.
Virology. 1966 Jun;29(2):303-9 PMID: 5949673
Induction of thymidine kinase by vaccinia-infected mouse fibroblasts.
J Mol Biol. 1963 Jan;6:22-33 PMID: 14033131
The purification fo four strains of poxvirus.
Virology. 1962 Sep;18:9-18 PMID: 14036977
DELETION OF THYMIDINE KINASE ACTIVITY FROM L CELLS RESISTANT TO BROMODEOXYURIDINE.
Exp Cell Res. 1963 Aug;31:297-312 PMID: 14056989
EFFECTS OF 5-FLUOROURACIL, ACTINOMYCIN D AND MITOMYCIN C ON THE INDUCTION OF THYMIDINE KINASE BY VACCINIA-INFECTED L-CELLS.
J Mol Biol. 1963 Nov;7:497-510 PMID: 14079589
THE INDUCTION AND REPRESSION OF THYMIDINE KINASE IN THE POXVIRUS-INFECTED HELA CELL.
Virology. 1963 Nov;21:383-9 PMID: 14081362
INHIBITION OF THYMIDINE KINASE FORMATION IN LM (TK-) CELLS SIMULTANEOUSLY INFECTED WITH VACCINIA AND A THYMIDINE KINASELESS VACCINIA MUTANT.
Virology. 1965 Jun;26:374-7 PMID: 14324004
Messenger RNA synthesis by a "coated" viral genome.
Proc Natl Acad Sci U S A. 1967 Feb;57(2):314-20 PMID: 16591471

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.16.1.210-213.1975

Published

1975-07-00

Pages

210-3

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC354651

Subset

External Links

PubMed source DOI: 10.1128/JVI.16.1.210-213.1975 PMC Full Text View journal details

Analysis Services

Control of thymidine kinase synthesis in IHD vaccinia virus-infected thymidine kinase-deficient LM cells.

Contact

WeChat Official Account