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PMID: 12426362 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA microarray analysis of the expression profile of Escherichia coli in response to treatment with 4,5-dihydroxy-2-cyclopenten-1-one.

Journal of bacteriology ·Vol. 184 ·No. 23 ·2002-12-00 ·Pages 6725-9

Phadtare S, Kato I, Inouye M

Abstract

We carried out DNA microarray-based global transcript profiling of Escherichia coli in response to 4,5-dihydroxy-2-cyclopenten-1-one to explore the manifestation of its antibacterial activity. We show that it has widespread effects in E. coli affecting genes encoding proteins involved in cell metabolism and membrane synthesis and functions. Genes belonging to the regulon involved in synthesis of Cys are upregulated. In addition, rpoS and RpoS-regulated genes responding to various stresses and a number of genes responding to oxidative stress are upregulated.

MeSH Terms
Cyclopentanes/pharmacology Escherichia coli/drug effects,growth & development,metabolism Escherichia coli Proteins/genetics,metabolism Gene Expression Profiling Gene Expression Regulation, Bacterial Oligonucleotide Array Sequence Analysis
Chemicals
4,5-dihydroxy-2-cyclopentan-1-one Cyclopentanes Escherichia coli Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Phadtare Sangita
Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA.
Kato Ikunoshin
Inouye Masayori
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.184.23.6725-6729.2002
Published
2002-12-00
Pages
6725-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC135408
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.184.23.6725-6729.2002 PMC Full Text View journal details
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