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PMID: 12414732 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Promoter proximal splice sites enhance transcription.

Genes & development ·Vol. 16 ·No. 21 ·2002-11-01 ·Pages 2792-9

Furger A, O'Sullivan JM, Binnie A, Lee BA, Proudfoot NJ

Abstract

Reconstruction of a gene with its introns removed results in reduced levels of cytoplasmic mRNA. This is partly explained by introns promoting the export of mRNA through coupling splicing to nuclear export processes. However, we show here that splicing signals can have a direct role in enhancing gene transcription. Removal of promoter proximal splice signals from a mammalian gene or the excision of introns from two different yeast genes results in a marked reduction in levels of nascent transcription, based on both nuclear run-on and direct image analysis. This further establishes that mRNA processing and transcription are tightly coupled mechanisms.

MeSH Terms

Genes, Fungal Genes, Viral HIV-1/genetics Promoter Regions, Genetic/genetics RNA Splice Sites/genetics RNA, Messenger/genetics Saccharomyces cerevisiae/genetics Transcription, Genetic

Chemicals

RNA Splice Sites RNA, Messenger

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Furger Andre

Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom.

O'Sullivan Justin M

Binnie Alexandra

Lee Barbara A

Proudfoot Nick J

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Article Info

Journal

Genes & development

Abbr.

Genes Dev

ISSN

0890-9369

DOI

10.1101/gad.983602

Published

2002-11-01

Pages

2792-9

Language

English

Region

United States

NLM ID

8711660

PMCID

PMC187476

Subset

External Links

PubMed source DOI: 10.1101/gad.983602 PMC Full Text View journal details

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