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PMID: 12414718 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of a new caged proton capable of inducing large pH jumps.

Biophysical journal ·Vol. 83 ·No. 5 ·2002-11-00 ·Pages 2864-71

Barth A, Corrie JE

Abstract

A new caged proton, 1-(2-nitrophenyl)ethyl sulfate (caged sulfate), is characterized by infrared spectroscopy and compared with a known caged, proton 2-hydroxyphenyl 1-(2-nitrophenyl)ethyl phosphate (caged HPP). In contrast to caged HPP, caged sulfate can induce large pH jumps and protonate groups that have pK values as low as 2.2. The photolysis mechanism of caged sulfate is analogous to that of P(3)-[1-(2-nitrophenyl)ethyl] ATP (caged ATP), and the photolysis efficiency is similar. The utility of this new caged compound for biological studies was demonstrated by its ability to drive the acid-induced conformational change of metmyoglobin. This transition from the native conformation to a partially unfolded form takes place near pH 4 and was monitored by near-UV absorption spectroscopy.

MeSH Terms

Biophysical Phenomena Biophysics Carboxylic Acids/chemistry Hydrogen/chemistry Hydrogen-Ion Concentration Light Metmyoglobin/chemistry Models, Chemical Nitrobenzenes/chemistry,pharmacology Organophosphates/chemistry Protein Conformation Protons Spectrophotometry Spectroscopy, Fourier Transform Infrared/methods Sulfates/chemistry Ultraviolet Rays

Chemicals

1-(2-nitrophenyl)ethyl sulfate Carboxylic Acids Nitrobenzenes Organophosphates Protons Sulfates Metmyoglobin 2-hydroxyphenyl 1-(2-nitrophenyl)ethyl phosphate Hydrogen

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Barth Andreas

Institut für Biophysik, Johann Wolfgang Goethe-Universität, D-60590 Frankfurt am Main, Germany.

Corrie John E T

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Article Info

Journal

Biophysical journal

Abbr.

Biophys J

ISSN

0006-3495

DOI

10.1016/S0006-3495(02)75295-8

Published

2002-11-00

Pages

2864-71

Language

English

Region

United States

NLM ID

0370626

PMCID

PMC1302370

Subset

External Links

PubMed source DOI: 10.1016/S0006-3495(02)75295-8 PMC Full Text View journal details

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Characterization of a new caged proton capable of inducing large pH jumps.

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