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PMID: 12391320 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

An approach to membrane protein structure without crystals.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 22 ·2002-10-29 ·Pages 14037-40

Sorgen PL, Hu Y, Guan L, Kaback HR, Girvin ME

Abstract

The lactose permease of Escherichia coli catalyzes coupled translocation of galactosides and H(+) across the cell membrane. It is the best-characterized member of the Major Facilitator Superfamily, a related group of membrane proteins with 12 transmembrane domains that mediate transport of various substrates across cell membranes. Despite decades of effort and their functional importance in all kingdoms of life, no high-resolution structures have been solved for any member of this family. However, extensive biochemical, genetic, and biophysical studies on lactose permease have established its transmembrane topology, secondary structure, and numerous interhelical contacts. Here we demonstrate that this information is sufficient to calculate a structural model at the level of helix packing or better.

MeSH Terms
Binding Sites Crystallization Escherichia coli/enzymology Escherichia coli Proteins Membrane Transport Proteins/chemistry Models, Molecular Monosaccharide Transport Proteins Nuclear Magnetic Resonance, Biomolecular/methods Protein Structure, Tertiary Symporters
Chemicals
Escherichia coli Proteins LacY protein, E coli Membrane Transport Proteins Monosaccharide Transport Proteins Symporters lactose permease
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sorgen Paul L
Biochemistry Department, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
Hu Yonglin
Guan Lan
Kaback H Ronald
Girvin Mark E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.182552199
Published
2002-10-29
Epub
2002-00-21
Pages
14037-40
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC137832
Subset
IM
Grants
NIGMS NIH HHS · F32 GM020504 · United States
NIDDK NIH HHS · R56 DK051131 · United States
NIDDK NIH HHS · DK51131:06 · United States
NIDDK NIH HHS · R01 DK051131 · United States
NIGMS NIH HHS · GM55371 · United States
NIGMS NIH HHS · F32 GM20504 · United States
NIGMS NIH HHS · R01 GM055371 · United States
External Links
PubMed source DOI: 10.1073/pnas.182552199 PMC Full Text View journal details
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