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PMID: 1238575 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Direct measurement of the membrane potential of Ehrlich ascites tumor cells: lack of effect of valinomycin and ouabain.

The Journal of membrane biology ·Vol. 23 ·No. 3-4 ·1975-00-00 ·Pages 349-65

Smith TC, Levinson C

Abstract

The membrane potential of Ehrlich ascites tumor cells and the effects of valinomycin and ouabain upon it have been determined. The membrane potential in control cells was 12.0 mV, inside negative. Neither valinomycin nor ouabain alone affected this value. However, valinomycin and ouabain in combination resulted in a slight hyperpolarization of the membrane. Concomitant determinations of cellular Na+, K+ and Cl- showed that valinomycin induced net losses of K+ and Cl- and a net gain in Na+ when compared to ouabain-inhibited cells. K+ permeability was increased by approximately 30% in the presence of valinomycin. In addition, valinomycin caused a rapid depletion of cellular ATP. Inhibition of Na/K transport by ouabain was without sparing effect on the rate of ATP depletion. Possible mechanisms for the electroneutral increase in K+ permeability induced by valinomycin are discussed.

MeSH Terms
Adenosine Triphosphate/metabolism Amphibians Animals Biological Transport, Active Cell Line Cell Membrane/metabolism,physiology Cell Membrane Permeability/drug effects Chlorides/metabolism Erythrocytes Membrane Potentials/drug effects Ouabain/pharmacology Potassium/metabolism Rotenone/pharmacology Sodium/metabolism Valinomycin/pharmacology
Chemicals
Chlorides Rotenone Valinomycin Ouabain Adenosine Triphosphate Sodium Potassium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Smith T C
Levinson C
References (21)
21 references, click to expand
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01870257
Published
1975-00-00
Pages
349-65
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01870257 View journal details
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