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PMID: 12383197 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cross-presentation of cell-associated antigens by CD8alpha+ dendritic cells is attributable to their ability to internalize dead cells.

Immunology ·Vol. 107 ·No. 2 ·2002-10-00 ·Pages 183-9

Schulz O, Reis e Sousa C

Abstract

In the mouse, cross-presentation is an exclusive property of the CD8alpha+ subset of dendritic cells (DC) but the basis for this selectivity remains unclear. Here we report that splenic CD8alpha+ DC are much superior to other DC subsets in internalizing dying cells in vitro. In contrast, CD8alpha+, CD8alpha- CD4+ and CD8alpha- CD4- DC subsets phagocytose bacteria or latex beads to a similar extent. Although CD8alpha+ DC are better than CD4+ DC at presenting ovalbumin (OVA)-loaded splenocytes to naïve OT-I T lymphocytes, CD4+ DC are better at presenting OVA-expressing Escherichia coli to the same T cells. In both cases, presentation is abrogated by lactacystin. These results show that both splenic CD8alpha+ and CD8alpha- DC can present exogenous antigens on major histocompatibility complex (MHC) class I via a proteasome-dependent pathway and suggest that the specialized cross-presenting function of CD8alpha+ DC is a result of their ability to endocytose dying cells rather than a unique pathway for handling endosomal contents.

MeSH Terms
Animals Antigen Presentation/immunology Antigens, Bacterial/immunology Apoptosis/immunology CD4 Antigens/analysis CD8 Antigens/analysis Cell Culture Techniques Dendritic Cells/immunology Escherichia coli/immunology Female Mice Mice, Inbred BALB C Mice, Inbred C57BL Microscopy, Video Ovalbumin/immunology Phagocytosis/immunology
Chemicals
Antigens, Bacterial CD4 Antigens CD8 Antigens CD8alpha antigen Ovalbumin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schulz Oliver
Immunobiology Laboratory, Cancer Research UK, London Research Institute, Lincoln's Inn Fields Laboratories, 44 Lincoln's Inn Fields, London WC2A 3PX, UK.
Reis e Sousa Caetano
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Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
DOI
10.1046/j.1365-2567.2002.01513.x
Published
2002-10-00
Pages
183-9
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1782783
Subset
IM
External Links
PubMed source DOI: 10.1046/j.1365-2567.2002.01513.x PMC Full Text View journal details
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