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PMID: 12381786 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ssdp proteins interact with the LIM-domain-binding protein Ldb1 to regulate development.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 22 ·2002-10-29 ·Pages 14320-5

Chen L, Segal D, Hukriede NA, Podtelejnikov AV, Bayarsaihan D, Kennison JA, Ogryzko VV, Dawid IB, Westphal H

Abstract

The LIM-domain-binding protein Ldb1 is a key factor in the assembly of transcriptional complexes involving LIM-homeodomain proteins and other transcription factors that regulate animal development. We identified Ssdp proteins (previously described as sequence-specific, single-stranded-DNA-binding proteins) as components of Ldb1-associated nuclear complexes in HeLa cells. Ssdp proteins are associated with Ldb1 in a variety of additional mammalian cell types. This association is specific, does not depend on the presence of nucleic acids, and is functionally significant. Genes encoding Ssdp proteins are well conserved in evolution from Drosophila to humans. Whereas the vertebrate Ssdp gene family has several closely related members, the Drosophila Ssdp gene is unique. In Xenopus, Ssdp encoded by Drosophila Ssdp or mouse Ssdp1 mRNA enhances axis induction by Ldb1 in conjunction with the LIM-homeobox gene Xlim1. Furthermore, we were able to demonstrate an interaction between Ssdp and Chip (the fly homolog of Ldb1) in Drosophila wing development. These findings indicate functional conservation of Ssdp as a cofactor of Ldb1 during invertebrate and vertebrate development.

MeSH Terms
3T3 Cells Amino Acid Sequence Animals COS Cells Chlorocebus aethiops DNA-Binding Proteins/genetics,metabolism Drosophila Proteins/genetics,metabolism Drosophila melanogaster/embryology,genetics,metabolism HeLa Cells Homeodomain Proteins/genetics,metabolism Humans LIM Domain Proteins LIM-Homeodomain Proteins Mice Mitochondrial Proteins Molecular Sequence Data Nuclear Proteins/genetics,metabolism Sequence Homology, Amino Acid Transcription Factors/genetics,metabolism Ubiquitin-Protein Ligases Vero Cells Xenopus/embryology Xenopus Proteins Zyxin
Chemicals
Chi protein, Drosophila DNA-Binding Proteins Drosophila Proteins Homeodomain Proteins LDB1 protein, human LHX1 protein, human LIM Domain Proteins LIM-Homeodomain Proteins Ldb1 protein, mouse Lhx1 protein, Xenopus Lhx1 protein, mouse Mitochondrial Proteins Nuclear Proteins SSBP1 protein, human SSBP3 protein, human Ssbp2 protein, mouse Ssbp3 protein, mouse Ssdp protein, Drosophila Transcription Factors XLdb1 protein, Xenopus Xenopus Proteins Zyx protein, Drosophila Zyxin STUB1 protein, human Ubiquitin-Protein Ligases
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Chen Lan
Laboratory of Mammalian Genes and Development, and Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20814, USA.
Segal Daniel
Hukriede Neil A
Podtelejnikov Alexandre V
Bayarsaihan Dashzeveg
Kennison James A
Ogryzko Vasily V
Dawid Igor B
Westphal Heiner
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.212532399
Published
2002-10-29
Epub
2002-00-15
Pages
14320-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC137882
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.212532399 PMC Full Text View journal details
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