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PMID: 12381660 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Histone chaperone ASF1 cooperates with the Brahma chromatin-remodelling machinery.

Genes & development ·Vol. 16 ·No. 20 ·2002-10-15 ·Pages 2621-6

Moshkin YM, Armstrong JA, Maeda RK, Tamkun JW, Verrijzer P, Kennison JA, Karch F

Abstract

De novo chromatin assembly into regularly spaced nucleosomal arrays is essential for eukaryotic genome maintenance and inheritance. The Anti-Silencing Function 1 protein (ASF1) has been shown to be a histone chaperone, participating in DNA-replication-coupled nucleosome assembly. We show that mutations in the Drosophila asf1 gene derepress silencing at heterochromatin and that the ASF1 protein has a cell cycle-specific nuclear and cytoplasmic localization. Furthermore, using both genetic and biochemical methods, we demonstrate that ASF1 interacts with the Brahma (SWI/SNF) chromatin-remodelling complex. These findings suggest that ASF1 plays a crucial role in both chromatin assembly and SWI/SNF-mediated chromatin remodelling.

MeSH Terms
Animals Cell Cycle Proteins/isolation & purification,physiology Chromosomal Proteins, Non-Histone/metabolism Crosses, Genetic Drosophila Proteins Drosophila melanogaster Eye Color/physiology Female Gene Deletion Gene Silencing Heterozygote In Vitro Techniques Male Molecular Chaperones/physiology Mutation Nucleosomes/metabolism Protein Transport Transcription Factors/metabolism
Chemicals
Cell Cycle Proteins Chromosomal Proteins, Non-Histone Drosophila Proteins Molecular Chaperones Nucleosomes SWI-SNF-B chromatin-remodeling complex Transcription Factors asf1 protein, Drosophila
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Moshkin Yuri M
Department of Zoology and Animal Biology, University of Geneva, 1211 Geneva 4, Switzerland.
Armstrong Jennifer A
Maeda Robert K
Tamkun John W
Verrijzer Peter
Kennison James A
Karch Francois
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.231202
Published
2002-10-15
Pages
2621-6
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC187460
Subset
IM
Grants
NIGMS NIH HHS · R01 GM049883 · United States
NIGMS NIH HHS · GM49883 · United States
External Links
PubMed source DOI: 10.1101/gad.231202 PMC Full Text View journal details
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