Abstract
Eight human isoforms of phosphoinositide 3-kinases (PI3Ks) exist, but their individual functions remain poorly understood. Here, we show that different human small cell lung carcinoma (SCLC) cell lines overexpress distinct subsets of class I(A) and II PI3Ks, which results in striking differences in the signalling cascades activated by stem cell factor (SCF). Over expression of class I(A) p85/p110alpha in SCLC cells increased SCF-stimulated protein kinase B (PKB) activation and cell growth, but did not affect extracellular signal-regulated kinase (Erk) or glycogen synthase kinase-3 (GSK-3). This effect was selective, since it was not observed in SCLC cell lines overexpressing p85/p110beta or p85/p110delta. The SCF receptor associated with both class I(A) p85 and class II PI3KC2beta, and both enzymes contributed to SCF-stimulated PKB activity. A dominant-negative PI3KC2beta blocked both PKB activation and SCLC cell growth in response to SCF. Together our data provide novel insights into the specificity and functional significance of PI3K signalling in human cancer.
MeSH Terms
Animals
COS Cells
Carcinoma, Small Cell
Cell Line
Chlorocebus aethiops
Enzyme Activation/drug effects
Growth Substances/pharmacology
Humans
Isoenzymes/genetics,metabolism
Lung Neoplasms
Phosphatidylinositol 3-Kinases/genetics,metabolism
Phosphorylation
Phosphotyrosine/metabolism
Protein Serine-Threonine Kinases
Proto-Oncogene Proteins/metabolism
Proto-Oncogene Proteins c-akt
Recombinant Proteins/metabolism
Transfection
Tumor Cells, Cultured
Chemicals
Growth Substances
Isoenzymes
Proto-Oncogene Proteins
Recombinant Proteins
Phosphotyrosine
Protein Serine-Threonine Kinases
Proto-Oncogene Proteins c-akt
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Arcaro Alexandre
Lung Cancer Biology Group, Division of Medicine, Imperial College Faculty of Medicine, Du Cane Road, London W12 0NN, UK. a.arcaro@ic.ac.uk
Khanzada Umme K
Vanhaesebroeck Bart
Tetley Teresa D
Waterfield Michael D
Seckl Michael J
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