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PMID: 12354931 Published · ppublish English Journal Article

Further evidence to support the melanocytic origin of MDA-MB-435.

Molecular pathology : MP ·Vol. 55 ·No. 5 ·2002-10-00 ·Pages 294-9

Ellison G, Klinowska T, Westwood RF, Docter E, French T, Fox JC

Abstract

Until recently, the cell line MDA-MB-435 was widely accepted as originating from a breast cancer. However, microarray derived data have suggested that this cell line may in fact originate from an occult melanoma. This study was designed to investigate this hypothesis further. Quantitative reverse transcription polymerase chain reaction and immunohistochemistry were used to investigate the tissue of origin of two sublines of MDA-MB-435 (MDA-MB-435 S and MDA-MB-435 HGF). The expression of a panel of genes typical of breast cells or melanocytes was analysed. The MDA-MD-435 cell lines expressed none of the genes characteristic of breast cancer cells but did express several genes commonly expressed by melanocytes. These results strongly suggest that MDA-MB-435 is indeed of melanoma origin.

MeSH Terms
Animals Biomarkers, Tumor/metabolism Breast Neoplasms/genetics,metabolism,pathology DNA, Complementary/genetics DNA, Neoplasm/genetics Female Gene Expression Gene Library Humans Immunoenzyme Techniques Melanocytes/pathology Melanoma/genetics,metabolism,pathology Mice Mice, SCID Neoplasm Proteins/metabolism Neoplasm Transplantation Reverse Transcriptase Polymerase Chain Reaction Transplantation, Heterologous Tumor Cells, Cultured/metabolism,pathology
Chemicals
Biomarkers, Tumor DNA, Complementary DNA, Neoplasm Neoplasm Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ellison G
AstraZeneca, Mereside, Alderley Park, Macclesfield, SK10 4TG, UK. gillian.ellison@aztrazeneca.com
Klinowska T
Westwood R F R
Docter E
French T
Fox J C
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Article Info
Journal
Molecular pathology : MP
Abbr.
Mol Pathol
ISSN
1366-8714
DOI
10.1136/mp.55.5.294
Email
gillian.ellison@aztrazeneca.com
Published
2002-10-00
Pages
294-9
Language
English
Region
England
NLM ID
9706282
PMCID
PMC1187258
Subset
IM
External Links
PubMed source DOI: 10.1136/mp.55.5.294 PMC Full Text
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