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PMID: 122510 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of Pseudomonas aeruginosa derepressed R-plasmids.

Journal of bacteriology ·Vol. 130 ·No. 2 ·1977-05-00 ·Pages 596-603

Chandler PM, Krishnapillai V

Abstract

A genetic study of conjugal transmissibility of two R-plasmids was undertaken in Pseudomonas aeruginosa. Conjugally derepressed mutants of the R-plasmids were isolated, and examination of 11 independent mutants revealed that 10 were recessive to the wild-type transfer repressor, whereas 1 mutant was cis dominant. Cross-repression was observed between the two R-plasmids, suggesting that they have functionally equivalent systems for regulating the expression of tra loci. The derepressed R-plasmid mutants exhibited several characteristics, in addition to derepressed transfer, that were not expressed by the parental plasmids. These included sensitivity to certain donor-specific phages, inhibition of multiplication of a transducing phage, and, in the one case examined, a high degree of entry exclusion. The coexpression of these different functions suggests that their respective genetic loci are controlled by the same regulatory system as that of tra, or else that they are part of the tra complex.

MeSH Terms
Bacteriophages/genetics Conjugation, Genetic Models, Genetic Mutation Pseudomonas aeruginosa/genetics R Factors Repressor Proteins Transduction, Genetic
Chemicals
Repressor Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chandler P M
Krishnapillai V
References (33)
33 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.130.2.596-603.1977
Published
1977-05-00
Pages
596-603
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC235257
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.130.2.596-603.1977 PMC Full Text View journal details
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