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PMID: 12239338 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Phosphorylation of the porcine reproductive and respiratory syndrome virus nucleocapsid protein.

Journal of virology ·Vol. 76 ·No. 20 ·2002-10-00 ·Pages 10569-76

Wootton SK, Rowland RR, Yoo D

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is a cytoplasmic RNA virus with the unique or unusual feature of having a nucleocapsid (N) protein that is specifically transported to the nucleolus of virus-infected cells. In this communication, we show that the N protein is a phosphoprotein. Phosphoamino acid analysis of authentic and recombinant N proteins demonstrated that serine residues were exclusively phosphorylated. The pattern of phosphorylated N protein cellular distribution in comparison with that of [(35)S]methionine-labeled N protein suggested that phosphorylation does not influence subcellular localization of the protein. Time course studies showed that phosphorylation occurred during, or shortly after, synthesis of the N protein and that the protein remained stably phosphorylated throughout the life cycle of the virus to the extent that phosphorylated N protein was found in the mature virion. Two-dimensional electrophoresis and acid-urea gel electrophoresis showed that one species of the N protein is predominant in virus-infected cells, suggesting that multiple phosphorylated isoforms of N do not exist.

MeSH Terms
Amino Acid Sequence Animals Binding Sites COS Cells Cell Nucleus/metabolism Chlorocebus aethiops Endoplasmic Reticulum/metabolism Kinetics Molecular Sequence Data Nucleocapsid Proteins/metabolism Phosphoproteins/metabolism Phosphorylation Porcine respiratory and reproductive syndrome virus/metabolism Swine
Chemicals
Nucleocapsid Proteins Phosphoproteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wootton Sarah K
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
Rowland Raymond R R
Yoo Dongwan
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.76.20.10569-10576.2002
Published
2002-10-00
Pages
10569-76
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC136587
Subset
IM
External Links
PubMed source DOI: 10.1128/jvi.76.20.10569-10576.2002 PMC Full Text View journal details
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