Home LiteratureArticle Details

PMID: 12237413 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A signal transduction protein cues proteolytic events critical to Caulobacter cell cycle progression.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 20 ·2002-10-01 ·Pages 13160-5

Hung DY, Shapiro L

Abstract

Temporally controlled proteolysis of the essential response regulator, CtrA, is critical for cell cycle progression in Caulobacter crescentus. CtrA binds to and silences the origin of replication in swarmer cells. The initiation of replication depends on the proteolysis of CtrA. We present evidence that DivK, an essential single-domain response regulator, contributes to the control of the G(1)-S transition by signaling the temporally controlled proteolysis of CtrA. In a divK-cs mutant at the restrictive temperature, the initiation of DNA replication is blocked because of the retention of CtrA. A shift of cells from restrictive to permissive temperature results in rapid degradation of CtrA, initiation of DNA replication, and the resumption of cell cycle progression, including the ordered expression of genes involved in chromosome replication and polar organelle biogenesis. CtrA binds to and regulates the promoters of two genes critical to its temporally controlled proteolysis, divK and clpP, providing a transcriptional feedback loop for the control of cell cycle progression.

MeSH Terms

Bacterial Proteins/genetics,metabolism Base Sequence Caulobacter/genetics,metabolism,physiology Cell Cycle Cell Survival DNA-Binding Proteins/genetics,metabolism Deoxyribonuclease I/metabolism Flow Cytometry Immunoblotting Models, Biological Molecular Sequence Data Mutation Oligonucleotide Array Sequence Analysis Phosphorylation Precipitin Tests Promoter Regions, Genetic Protein Binding RNA, Messenger/metabolism Replication Origin Signal Transduction Temperature Time Factors Transcription Factors/genetics,metabolism

Chemicals

Bacterial Proteins CtrA protein, Caulobacter DNA-Binding Proteins DivK protein, Caulobacter crescentus RNA, Messenger Transcription Factors Deoxyribonuclease I

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Hung Dean Y

Department of Developmental Biology, Beckman Center, Stanford University School of Medicine, Stanford, CA 94305, USA.

Shapiro Lucy

References (19)

19 references, click to expand

A novel bacterial tyrosine kinase essential for cell division and differentiation.
Proc Natl Acad Sci U S A. 1999 Nov 9;96(23):13068-73 PMID: 10557274
Cell cycle-dependent polar localization of an essential bacterial histidine kinase that controls DNA replication and cell division.
Cell. 1999 Apr 2;97(1):111-20 PMID: 10199407
Signal transduction mechanisms in Caulobacter crescentus development and cell cycle control.
FEMS Microbiol Rev. 2000 Apr;24(2):177-91 PMID: 10717313
Global analysis of the genetic network controlling a bacterial cell cycle.
Science. 2000 Dec 15;290(5499):2144-8 PMID: 11118148
Dynamic localization of a cytoplasmic signal transduction response regulator controls morphogenesis during the Caulobacter cell cycle.
Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):4095-100 PMID: 11274434
Genes directly controlled by CtrA, a master regulator of the Caulobacter cell cycle.
Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4632-7 PMID: 11930012
Pseudoreversion analysis indicates a direct role of cell division genes in polar morphogenesis and differentiation in Caulobacter crescentus.
Genetics. 1991 Nov;129(3):623-30 PMID: 1752411
A histidine protein kinase is involved in polar organelle development in Caulobacter crescentus.
Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):630-4 PMID: 8421698
Requirement of the carboxyl terminus of a bacterial chemoreceptor for its targeted proteolysis.
Science. 1993 Mar 19;259(5102):1754-7 PMID: 8456303
An essential single domain response regulator required for normal cell division and differentiation in Caulobacter crescentus.
EMBO J. 1995 Aug 15;14(16):3915-24 PMID: 7664732
Cell cycle control by an essential bacterial two-component signal transduction protein.
Cell. 1996 Jan 12;84(1):83-93 PMID: 8548829
Cell type-specific phosphorylation and proteolysis of a transcriptional regulator controls the G1-to-S transition in a bacterial cell cycle.
Cell. 1997 Aug 8;90(3):415-24 PMID: 9267022
Negative control of bacterial DNA replication by a cell cycle regulatory protein that binds at the chromosome origin.
Proc Natl Acad Sci U S A. 1998 Jan 6;95(1):120-5 PMID: 9419339
An essential, multicomponent signal transduction pathway required for cell cycle regulation in Caulobacter.
Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1443-8 PMID: 9465034
An essential protease involved in bacterial cell-cycle control.
EMBO J. 1998 Oct 1;17(19):5658-69 PMID: 9755166
The CtrA response regulator mediates temporal control of gene expression during the Caulobacter cell cycle.
J Bacteriol. 1999 Apr;181(8):2430-9 PMID: 10198005
Cell cycle-dependent degradation of a flagellar motor component requires a novel-type response regulator.
Mol Microbiol. 1999 Apr;32(2):379-91 PMID: 10231493
Feedback control of a master bacterial cell-cycle regulator.
Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6648-53 PMID: 10359766
Differential localization of two histidine kinases controlling bacterial cell differentiation.
Mol Cell. 1999 Nov;4(5):683-94 PMID: 10619016

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.202495099

Published

2002-10-01

Epub

2002-00-17

Pages

13160-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC130603

Subset

Grants

PHS HHS · 3206/512M2 · United States

External Links

PubMed source DOI: 10.1073/pnas.202495099 PMC Full Text View journal details

Analysis Services

A signal transduction protein cues proteolytic events critical to Caulobacter cell cycle progression.

Contact

WeChat Official Account