Subcellular Compartmentation of the 4-Aminobutyrate Shunt in Protoplasts from Developing Soybean Cotyledons.

Plant physiology ·Vol. 108 ·No. 1 ·1995-05-00 ·Pages 99-103

Breitkreuz KE, Shelp BJ

Abstract

The subcellular localization of enzymes involved in the 4-ami-nobutyrate shunt was investigated in protoplasts prepared from developing soybean [Glycine max (L.) Merrill cv Maple Arrow] cotyledons. Protoplast lysate was fractionated by differential and continuous Percoll-gradient centrifugation to separate organelle fractions. Glutamate decarboxylase (EC 4.1.1.15) was found exclusively in the cytosol, whereas 4-aminobutyrate:pyruvate transami-nase (EC 2.6.1.19) and succinic semialdehyde dehydrogenase (EC 1.2.1.16) were associated exclusively with the mitochondrial fractions. Mitochondrial fractions also catabolized [U-14C]4-aminobu-tyrate to labeled succinate.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Breitkreuz K. E.

Department of Horticultural Science and Interdepartmental Plant Physiology Program, University of Guelph, Guelph, Ontario, Canada N1G 2W1.

Shelp B. J.

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Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

1532-2548

DOI

10.1104/pp.108.1.99

Published

1995-05-00

Pages

99-103

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC157309

External Links

PubMed source DOI: 10.1104/pp.108.1.99 PMC Full Text View journal details

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Subcellular Compartmentation of the 4-Aminobutyrate Shunt in Protoplasts from Developing Soybean Cotyledons.

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