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PMID: 12205292 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

M-FISH analysis shows that complex chromosome aberrations induced by alpha -particle tracks are cumulative products of localized rearrangements.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 19 ·2002-09-17 ·Pages 12167-72

Anderson RM, Stevens DL, Goodhead DT

Abstract

Complex chromosome aberrations are characteristically induced after exposure to low doses of densely ionizing radiation, but little is understood about their formation. To address this issue, we irradiated human peripheral blood lymphocytes in vitro with 0.5 Gy densely ionizing alpha-particles (mean of 1 alpha-particle/cell) and analyzed the chromosome aberrations produced by using 24-color multiplex fluorescence in situ hybridization (M-FISH). Our data suggest that complex formation is a consequence of direct nuclear alpha-particle traversal and show that the likely product of illegitimate repair of damage from a single alpha-particle is a single complex exchange. From an assessment of the "cycle structure" of each complex exchange we predict alpha-particle-induced damage to be repaired at specific localized sites, and complexes to be formed as cumulative products of this repair.

MeSH Terms
Alpha Particles/adverse effects Biophysical Phenomena Biophysics Chromosome Aberrations/radiation effects Chromosome Painting DNA Repair Gene Rearrangement/radiation effects Humans In Vitro Techniques Karyotyping Lymphocytes/metabolism,radiation effects,ultrastructure Models, Genetic
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Anderson Rhona M
Medical Research Council Radiation and Genome Stability Unit, Harwell, Didcot, Oxfordshire OX11 0RD, United Kingdom. r.anderson@har.mrc.ac.uk
Stevens David L
Goodhead Dudley T
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.182426799
Email
r.anderson@har.mrc.ac.uk
Published
2002-09-17
Epub
2002-00-30
Pages
12167-72
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC129416
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.182426799 PMC Full Text View journal details
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