Technical improvement to prevent DNA degradation of enteric pathogens in pulsed-field gel electrophoresis.

Journal of clinical microbiology ·Vol. 40 ·No. 9 ·2002-09-00 ·Pages 3497-8

Koort JM, Lukinmaa S, Rantala M, Unkila E, Siitonen A

Abstract

This study used a modified pulsed-field gel electrophoresis (PFGE) method with HEPES as a running buffer to prevent electrophoresis-related DNA degradation of nine Salmonella enterica subsp. enterica serovar Ohio, seven Salmonella serovar Newport, and two enterohemorrhagic Escherichia coli (non-O157) strains. All strains yielded identifiable bands with this method in contrast to a commonly applied PFGE method using Tris buffer.

MeSH Terms

Bacterial Typing Techniques/methods Buffers DNA, Bacterial/chemistry Electrophoresis, Gel, Pulsed-Field/methods Escherichia coli/classification,genetics HEPES Humans Salmonella enterica/classification,genetics

Chemicals

Buffers DNA, Bacterial HEPES

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Koort Joanna M K

Laboratory of Enteric Pathogens, National Public Health Institute, Helsinki, Finland.

Lukinmaa Susanna

Rantala Marjatta

Unkila Erja

Siitonen Anja

References (12)

12 references, click to expand

Achieving 100% typeability of Pseudomonas aeruginosa by pulsed-field gel electrophoresis.
J Clin Microbiol. 2000 Jan;38(1):464-5 PMID: 10618146
Molecular epidemiology of an outbreak caused by Salmonella enterica serovar Newport in Finland and the United Kingdom.
Epidemiol Infect. 2000 Apr;124(2):185-92 PMID: 10813141
Pulsed-field gel electrophoresis of degradation-sensitive DNAs from Clostridium difficile PCR ribotype 1 strains.
J Clin Microbiol. 2000 Jul;38(7):2791-2 PMID: 10878091
Diversity of strains of Salmonella enterica serotype enteritidis from English poultry farms assessed by multiple genetic fingerprinting.
J Clin Microbiol. 2001 Jan;39(1):154-61 PMID: 11136764
Fluorescent amplified-fragment length polymorphism subtyping of the Salmonella enterica serovar enteritidis phage type 4 clone complex.
J Clin Microbiol. 2001 Jan;39(1):201-6 PMID: 11136771
Salmonella enteritidis phage types 1 and 4: pheno- and genotypic epidemiology of recent outbreaks in Finland.
J Clin Microbiol. 1999 Jul;37(7):2176-82 PMID: 10364582
Tris-dependent site-specific cleavage of Streptomyces lividans DNA.
FEMS Microbiol Lett. 1992 Sep 15;75(2-3):247-52 PMID: 1398041
Evaluation of DNA fingerprinting by PFGE as an epidemiologic tool for Salmonella infections.
Microbiol Immunol. 1995;39(9):673-6 PMID: 8577280
Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day.
J Clin Microbiol. 1997 Nov;35(11):2977-80 PMID: 9350772
High-resolution genotyping of Salmonella strains by AFLP-fingerprinting.
Lett Appl Microbiol. 1998 Feb;26(2):131-5 PMID: 9569696
Molecular fingerprinting defines a strain of Salmonella enterica serotype Anatum responsible for an international outbreak associated with formula-dried milk.
Epidemiol Infect. 1998 Oct;121(2):289-93 PMID: 9825779
Automated ribotyping and random amplified polymorphic DNA analysis for molecular typing of Salmonella enteritidis and Salmonella typhimurium strains isolated in Italy.
J Appl Microbiol. 2001 Nov;91(5):780-5 PMID: 11722654

Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/JCM.40.9.3497-3498.2002

Published

2002-09-00

Pages

3497-8

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC130692

Subset

External Links

PubMed source DOI: 10.1128/JCM.40.9.3497-3498.2002 PMC Full Text View journal details

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Technical improvement to prevent DNA degradation of enteric pathogens in pulsed-field gel electrophoresis.

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