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PMID: 12196554 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Reversible translocation and activity-dependent localization of the calcium-myristoyl switch protein VILIP-1 to different membrane compartments in living hippocampal neurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 22 ·No. 17 ·2002-09-01 ·Pages 7331-9

Spilker C, Dresbach T, Braunewell KH

Abstract

Visinin-like protein-1 (VILIP-1) belongs to the family of neuronal calcium sensor (NCS) proteins, a neuronal subfamily of EF-hand [corrected] calcium-binding proteins that are myristoylated at their N termini. NCS proteins are discussed to play roles in calcium-dependent signal transduction of physiological and pathological processes in the CNS. The calcium-dependent membrane association, the so-called calcium-myristoyl switch, localizes NCS proteins to a distinct cellular signaling compartment and thus may be a critical mechanism for the coordinated regulation of signaling cascades. To study whether the biochemically defined calcium-myristoyl switch of NCS proteins can occur in living neuronal cells, the reversible and stimulus-dependent translocation of green fluorescent protein (GFP)-tagged VILIP-1 to subcellular targets was examined by fluorescence microscopy in transfected cell lines and hippocampal primary neurons. In transiently transfected NG108-15 and COS-7 cells, a translocation of diffusely distributed VILIP-1-GFP but not of myristoylation-deficient VILIP-1-GFP to the plasma membrane and to intracellular targets, such as Golgi membranes, occurred after raising the intracellular calcium concentration with a calcium ionophore. The observed calcium-dependent localization was completely reversed after depletion of intracellular calcium by EGTA. Interestingly, a fast and reversible translocation of VILIP-1-GFP and translocation of endogenous VILIP-1 to specialized membrane structures was also observed after a depolarizing stimulus or activation of glutamate receptors in hippocampal neurons. These results show for the first time the reversibility and stimulus-dependent occurrence of the calcium-myristoyl switch in living neurons, suggesting a physiological role as a signaling mechanism of NCS proteins, enabling them to activate specific targets localized in distinct membrane compartments.

MeSH Terms
Animals COS Cells Calcium/metabolism Calcium-Binding Proteins/genetics,metabolism Cell Compartmentation/physiology Cell Line Green Fluorescent Proteins Hippocampus/metabolism Internet Intracellular Fluid/metabolism Intracellular Membranes/metabolism Luminescent Proteins/genetics Mice Microscopy, Fluorescence Microscopy, Video Myristates/metabolism Nerve Tissue Proteins/genetics,metabolism Neurocalcin Neurons/cytology,metabolism Protein Transport/physiology Rats Receptors, Calcium-Sensing Recombinant Fusion Proteins/genetics,metabolism Signal Transduction/physiology Transfection Video Recording
Chemicals
Calcium-Binding Proteins Luminescent Proteins Myristates Nerve Tissue Proteins Neurocalcin Receptors, Calcium-Sensing Recombinant Fusion Proteins Vsnl1 protein, mouse Vsnl1 protein, rat Green Fluorescent Proteins Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Spilker Christina
Neuroscience Research Center-Institute for Physiology of the Charite, Humboldt University Berlin, Signal Transduction Research Group, D-10117 Berlin, Germany.
Dresbach Thomas
Braunewell Karl-Heinz
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
Published
2002-09-01
Pages
7331-9
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6757958
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source PMC Full Text View journal details
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