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PMID: 12193787 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Distinct modes of signal recognition particle interaction with the ribosome.

Science (New York, N.Y.) ·Vol. 297 ·No. 5585 ·2002-08-23 ·Pages 1345-8

Pool MR, Stumm J, Fulga TA, Sinning I, Dobberstein B

Abstract

Signal recognition particle (SRP), together with its receptor (SR), mediates the targeting of ribosome-nascent chain complexes to the endoplasmic reticulum. Using protein cross-linking, we detected distinct modes in the binding of SRP to the ribosome. During signal peptide recognition, SRP54 is positioned at the exit site close to ribosomal proteins L23a and L35. When SRP54 contacts SR, SRP54 is rearranged such that it is no longer close to L23a. This repositioning may allow the translocon to dock with the ribosome, leading to insertion of the signal peptide into the translocation channel.

MeSH Terms
Animals Centrifugation, Density Gradient Cross-Linking Reagents Dogs Guanosine Diphosphate/metabolism,pharmacology Guanosine Triphosphate/metabolism Guanylyl Imidodiphosphate/metabolism,pharmacology Models, Molecular Plant Proteins/chemistry,metabolism Precipitin Tests Prolactin/genetics,metabolism Protein Binding Protein Precursors/genetics,metabolism Protein Sorting Signals Protein Transport Receptors, Cytoplasmic and Nuclear/chemistry,metabolism Receptors, Peptide/chemistry,metabolism Ribosomal Proteins/chemistry,metabolism Ribosomes/metabolism Saccharomyces cerevisiae Proteins Signal Recognition Particle/chemistry,metabolism Succinimides
Chemicals
Cross-Linking Reagents Plant Proteins Protein Precursors Protein Sorting Signals RPL35A protein, S cerevisiae Receptors, Cytoplasmic and Nuclear Receptors, Peptide Ribosomal Proteins SRP54 protein, S cerevisiae Saccharomyces cerevisiae Proteins Signal Recognition Particle Succinimides signal peptide receptor Guanosine Diphosphate Guanylyl Imidodiphosphate preprolactin Guanosine Triphosphate Prolactin disuccinimidyl suberate
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Pool Martin R
Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), D-69120 Heidelberg, Germany. mrp@zmbh.uni-heidelberg.de
Stumm Joachim
Fulga Tudor A
Sinning Irmgard
Dobberstein Bernhard
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
1095-9203
Published
2002-08-23
Pages
1345-8
Language
English
Region
United States
NLM ID
0404511
Subset
IM
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