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PMID: 12181346 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dissection of transient oxidative stress response in Saccharomyces cerevisiae by using DNA microarrays.

Molecular biology of the cell ·Vol. 13 ·No. 8 ·2002-08-00 ·Pages 2783-94

Koerkamp MG, Rep M, Bussemaker HJ, Hardy GP, Mul A, Piekarska K, Szigyarto CA, De Mattos JM, Tabak HF

Abstract

Yeast cells were grown in glucose-limited chemostat cultures and forced to switch to a new carbon source, the fatty acid oleate. Alterations in gene expression were monitored using DNA microarrays combined with bioinformatics tools, among which was included the recently developed algorithm REDUCE. Immediately after the switch to oleate, a transient and very specific stress response was observed, followed by the up-regulation of genes encoding peroxisomal enzymes required for fatty acid metabolism. The stress response included up-regulation of genes coding for enzymes to keep thioredoxin and glutathione reduced, as well as enzymes required for the detoxification of reactive oxygen species. Among the genes coding for various isoenzymes involved in these processes, only a specific subset was expressed. Not the general stress transcription factors Msn2 and Msn4, but rather the specific factor Yap1p seemed to be the main regulator of the stress response. We ascribe the initiation of the oxidative stress response to a combination of poor redox flux and fatty acid-induced uncoupling of the respiratory chain during the metabolic reprogramming phase.

MeSH Terms
Active Transport, Cell Nucleus Algorithms DNA-Binding Proteins/genetics,metabolism Gene Expression Profiling Gene Expression Regulation, Fungal Genes, Fungal Oleic Acid/metabolism Oligonucleotide Array Sequence Analysis Oxidation-Reduction Oxidative Stress Peroxisomes/metabolism RNA, Messenger/genetics,metabolism Reactive Oxygen Species/metabolism Recombinant Fusion Proteins/genetics,metabolism Saccharomyces cerevisiae/genetics,physiology Saccharomyces cerevisiae Proteins/genetics,metabolism Time Factors Transcription Factors/genetics,metabolism Transcription, Genetic
Chemicals
DNA-Binding Proteins MSN2 protein, S cerevisiae RNA, Messenger Reactive Oxygen Species Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Transcription Factors Oleic Acid
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Koerkamp Marian Groot
Department of Biochemistry, Academic Medical Center, University of Amsterdam, The Netherlands.
Rep Martijn
Bussemaker Harmen J
Hardy Guy P M A
Mul Adri
Piekarska Kasia
Szigyarto Cristina Al-Khalili
De Mattos Joost M Teixeira
Tabak Henk F
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e02-02-0075
Published
2002-08-00
Pages
2783-94
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC117942
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.e02-02-0075 PMC Full Text View journal details
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