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PMID: 12181289 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Excitation-contraction coupling and sarcoplasmic reticulum function in mechanically skinned fibres from fast skeletal muscles of aged mice.

The Journal of physiology ·Vol. 543 ·No. Pt 1 ·2002-08-15 ·Pages 169-76

Plant DR, Lynch GS

Abstract

Ageing is generally associated with a decline in skeletal muscle mass and strength, and a slowing of muscle contraction, factors that impact upon the quality of life for the elderly. Alterations in Ca2+ handling are thought to contribute to these age-related changes in muscle contractility, yet the effects of ageing on sarcoplasmic reticulum (SR) Ca2+ handling and the Ca2+ transport system remain unresolved. We used mechanically skinned single fibres from the fast twitch extensor digitorum longus (EDL) muscles from young (4-month-old) and old (27- to 28-month-old) mice to test the hypothesis that the age-related changes in skeletal muscle contractility, especially the slower rate of contraction, are due to changes intrinsic to the muscle fibres. There were no age-related differences in the peak height of depolarization-induced contractile response (DICR) or the number of DICRs elicited before rundown (DICR < 50 % of initial). The time taken to reach peak DICR (TPDICR) was approximately12 % slower in single muscle fibres from old compared with young mice (P < 0.05). The rate of relaxation following DICR was not different in young and old mice. Examination of SR function demonstrated that SR Ca2+ reloading in Ca2+ -depleted skinned fibres was not different in young and old mice, nor was there any age-related difference in Ca2+ leak from the SR. However, low [caffeine] contracture in fibres from old mice was only half of that observed in fibres from young mice (P < 0.05), indicating a lower sensitivity of the SR Ca2+ release channel (CRC) to caffeine. We found no difference in maximum Ca2+ -activated force (P(o)) or specific force (sP(o); P(o) corrected for cross-sectional area) in EDL muscle fibres from young and old mice. Impaired excitation-contraction (E-C) coupling and a decrease in SR CRC function are mechanisms which are likely to contribute to the overall slowing of muscle contraction with age.

MeSH Terms
Aging/physiology Animals Caffeine/pharmacology Calcium/metabolism In Vitro Techniques Male Membrane Potentials/drug effects,physiology Mice Mice, Inbred C57BL Muscle Contraction/drug effects,physiology Muscle Fibers, Fast-Twitch/physiology Phosphodiesterase Inhibitors/pharmacology Sarcoplasmic Reticulum/physiology
Chemicals
Phosphodiesterase Inhibitors Caffeine Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Plant David R
Department of Physiology, The University of Melbourne, Victoria 3010, Australia.
Lynch Gordon S
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.2002.022418
Published
2002-08-15
Pages
169-76
Language
English
Region
England
NLM ID
0266262
PMCID
PMC2290492
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.2002.022418 PMC Full Text View journal details
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