Efficient delivery of siRNA for inhibition of gene expression in postnatal mice.

Nature genetics ·Vol. 32 ·No. 1 ·2002-09-00 ·Pages 107-8

Lewis DL, Hagstrom JE, Loomis AG, Wolff JA, Herweijer H

Abstract

It has recently been shown that RNA interference can be induced in cultured mammalian cells by delivery of short interfering RNAs (siRNAs). Here we describe a method for efficient in vivo delivery of siRNAs to organs of postnatal mice and demonstrate effective and specific inhibition of transgene expression in a variety of organs.

MeSH Terms

Animals Animals, Newborn Cnidaria Coleoptera Gene Expression Regulation Gene Transfer Techniques Green Fluorescent Proteins Liver/metabolism Luciferases/genetics Luminescent Proteins/genetics Mice Plasmids RNA, Small Interfering RNA, Untranslated/administration & dosage Transgenes

Chemicals

Luminescent Proteins RNA, Small Interfering RNA, Untranslated Green Fluorescent Proteins Luciferases

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Lewis David L

Mirus Corporation, 505 South Rosa Road, Madison, Wisconsin 53719, USA. davel@genetransfer.com

Hagstrom James E

Loomis Aaron G

Wolff Jon A

Herweijer Hans

Article Info

Journal

Nature genetics

Abbr.

Nat Genet

ISSN

1061-4036

DOI

10.1038/ng944

davel@genetransfer.com

Published

2002-09-00

Epub

2002-00-29

Pages

107-8

Language

English

Region

United States

NLM ID

9216904

Subset

External Links

PubMed source DOI: 10.1038/ng944 View journal details

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