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PMID: 1212228 Published · ppublish English Journal Article

Regulation of ribonucleic acid synthesis by polyamines. Reversal by spermine of inhibition by methylglyoxal bis(guanylhydrazone) of ribonucleic acid synthesis and histone acetylation in rabbit heart.

The Biochemical journal ·Vol. 152 ·No. 1 ·1975-10-00 ·Pages 91-8

Caldarera CM, Casti A, Guarnier C, Moruzzi G

Abstract

The relationship between polyamines and RNA synthesis was studied by considering the action of spermine on histone acetylation in perfused heart. In addition, the effect of methylglyoxal bis(guanylhydrazone), inhibitor of putrescine-activated S-adenosylmethionine decarboxylase activity, on RNA and polyamine specific radioactivity and on acetylation of histone fractions was also investigated in perfused heart. Different concentrations of spermine and/or methylglyoxas bis(guanylhydrazone) were injected into the heart, 15 min after beginning the perfusion. The results demonstrate that spermine stimulates the specific radioactivity of RNA of subcellular fractions. Acetylation of the arginine-rich histone fractions, involved in the regulation of RNA transcription, is enhanced by spermine. The perfusion with methylglyoxal bis(guanylhydrazone) causes a decrease in the specific radioactivity of polyamines and RNA, and in acetylation of histone fractions. However, spermine is able to reverse the methylglyoxal bis(guanylhydrazone) inhibition when injected simultaneously. From these results we may assume a possible role for spermine in the regulation of RNA transcription.

MeSH Terms
Acetylation Animals Guanidines/pharmacology Heart/drug effects Histones/metabolism In Vitro Techniques Mitoguazone/pharmacology Myocardium/metabolism RNA/biosynthesis Rabbits Spermine/pharmacology Time Factors
Chemicals
Guanidines Histones Spermine RNA Mitoguazone
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Caldarera C M
Casti A
Guarnier C
Moruzzi G
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41 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1520091
Published
1975-10-00
Pages
91-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1172443
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1520091 PMC Full Text View journal details
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