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PMID: 12110167 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

tej defines a role for poly(ADP-ribosyl)ation in establishing period length of the arabidopsis circadian oscillator.

Developmental cell ·Vol. 3 ·No. 1 ·2002-07-00 ·Pages 51-61

Panda S, Poirier GG, Kay SA

Abstract

In a genetic screen for altered circadian period length in Arabidopsis, we isolated a mutant with a long free-running period. The tej mutation acts independently of light quality and quantity. It affects clock-controlled transcription of genes in Arabidopsis and alters the timing of the photoperiod-dependent transition from vegetative growth to flowering. Map-based cloning of TEJ identified a poly(ADP-ribose) glycohydrolase (PARG). An inhibitor of poly(ADP-ribosyl)ation rescued the period phenotype of tej mutant and shortened the period length of wild-type plants. Posttranslational poly(ADP-ribosyl)ation of an oscillator component may contribute to setting the period length of the Arabidopsis central oscillator.

MeSH Terms
Arabidopsis/cytology,genetics,metabolism Arabidopsis Proteins/genetics,isolation & purification,metabolism Benzamides/pharmacology Biological Clocks/genetics Circadian Rhythm/genetics Cloning, Molecular Enzyme Inhibitors/pharmacology Gene Expression Regulation, Plant/drug effects,physiology Glycoside Hydrolases/genetics,isolation & purification,metabolism Molecular Sequence Data Mutation/genetics Periodicity Phenotype Photic Stimulation Photoperiod Poly Adenosine Diphosphate Ribose/genetics,metabolism Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid
Chemicals
Arabidopsis Proteins Benzamides Enzyme Inhibitors Poly Adenosine Diphosphate Ribose 3-aminobenzamide Glycoside Hydrolases poly ADP-ribose glycohydrolase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Panda Satchidananda
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Poirier Guy G
Kay Steve A
Article Info
Journal
Developmental cell
Abbr.
Dev Cell
ISSN
1534-5807
Published
2002-07-00
Pages
51-61
Language
English
Region
United States
NLM ID
101120028
Subset
IM
Grants
NIGMS NIH HHS · GM56006 · United States
Databases
GENBANK
AF394690
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