RNA interference in adult mice.

Nature ·Vol. 418 ·No. 6893 ·2002-07-04 ·Pages 38-9

McCaffrey AP, Meuse L, Pham TT, Conklin DS, Hannon GJ, Kay MA

Abstract

RNA interference is an evolutionarily conserved surveillance mechanism that responds to double-stranded RNA by sequence-specific silencing of homologous genes. Here we show that transgene expression can be suppressed in adult mice by synthetic small interfering RNAs and by small-hairpin RNAs transcribed in vivo from DNA templates. We also show the therapeutic potential of this technique by demonstrating effective targeting of a sequence from hepatitis C virus by RNA interference in vivo.

MeSH Terms

Aging/genetics Animals Gene Silencing Genes, Reporter/genetics Hepacivirus/genetics Humans Liver/metabolism Luciferases/biosynthesis,genetics Mice RNA, Double-Stranded/chemistry,genetics,metabolism RNA, Small Interfering RNA, Untranslated/chemistry,genetics,metabolism RNA, Viral/genetics,metabolism Recombinant Fusion Proteins/biosynthesis,genetics Substrate Specificity Transgenes/genetics Viral Nonstructural Proteins/biosynthesis,genetics

Chemicals

NS-5 protein, hepatitis C virus RNA, Double-Stranded RNA, Small Interfering RNA, Untranslated RNA, Viral Recombinant Fusion Proteins Viral Nonstructural Proteins Luciferases

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

McCaffrey Anton P

Department of Pediatrics, Stanford University School of Medicine, Stanford, California 94305-5208, USA.

Meuse Leonard

Pham Thu-Thao T

Conklin Douglas S

Hannon Gregory J

Kay Mark A

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/418038a

Published

2002-07-04

Pages

38-9

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/418038a View journal details

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RNA interference in adult mice.

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